Impact of uterine macrophage phenotype on placental retention in dairy cows.
Bovine monocytes
M2 macrophages
Retained placenta
STAT3
Uterine caruncles
Journal
Theriogenology
ISSN: 1879-3231
Titre abrégé: Theriogenology
Pays: United States
ID NLM: 0421510
Informations de publication
Date de publication:
15 Mar 2019
15 Mar 2019
Historique:
received:
22
08
2018
revised:
26
12
2018
accepted:
11
01
2019
pubmed:
30
1
2019
medline:
23
2
2019
entrez:
30
1
2019
Statut:
ppublish
Résumé
Reproductive diseases affect 25% of dairy cows in the US and often develop from retention of the placenta. It is well established that expulsion of the placenta is a highly regulated inflammatory process, but the mechanisms by which dysregulation of uterine immune responses impair this process are poorly understood. In healthy non-ruminants, pro-inflammatory M1 macrophages are predominant in uterine tissue after parturition. However, macrophage phenotype in the postpartum bovine uterus is unknown. Our study compared macrophage phenotypes in the uterine caruncles of multiparous dairy cows that during the first day postpartum either retained (RET, n = 5) or had normal expulsion (NOR, n = 5) of placenta. Immune cells were sorted magnetically from the caruncular endometrial cell fraction using the CD172a marker and monocyte/macrophage population was characterized using flow cytometry. Transcriptional and protein expression studies were performed on uterine caruncles. Compared to NOR, RET samples showed a lower CD14
Identifiants
pubmed: 30695743
pii: S0093-691X(18)30676-9
doi: 10.1016/j.theriogenology.2019.01.011
pii:
doi:
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
145-152Informations de copyright
Copyright © 2019 Elsevier Inc. All rights reserved.