Induction of human pluripotent stem cells into kidney tissues by synthetic mRNAs encoding transcription factors.
Biomarkers
Cell Culture Techniques
Cell Differentiation
/ genetics
Cell Lineage
/ genetics
Fluorescent Antibody Technique
Homeodomain Proteins
/ genetics
Humans
Immunophenotyping
Induced Pluripotent Stem Cells
/ cytology
Kidney
/ cytology
Models, Biological
Nephrons
Nerve Tissue Proteins
/ genetics
Pluripotent Stem Cells
/ cytology
RNA, Messenger
/ genetics
Transcription Factors
/ genetics
Journal
Scientific reports
ISSN: 2045-2322
Titre abrégé: Sci Rep
Pays: England
ID NLM: 101563288
Informations de publication
Date de publication:
29 01 2019
29 01 2019
Historique:
received:
09
03
2018
accepted:
05
12
2018
entrez:
31
1
2019
pubmed:
31
1
2019
medline:
13
8
2020
Statut:
epublish
Résumé
The derivation of kidney tissues from human pluripotent stem cells (hPSCs) and its application for replacement therapy in end-stage renal disease have been widely discussed. Here we report that consecutive transfections of two sets of synthetic mRNAs encoding transcription factors can induce rapid and efficient differentiation of hPSCs into kidney tissues, termed induced nephron-like organoids (iNephLOs). The first set - FIGLA, PITX2, ASCL1 and TFAP2C, differentiated hPSCs into SIX2
Identifiants
pubmed: 30696889
doi: 10.1038/s41598-018-37485-8
pii: 10.1038/s41598-018-37485-8
pmc: PMC6351687
doi:
Substances chimiques
Biomarkers
0
Homeodomain Proteins
0
Nerve Tissue Proteins
0
RNA, Messenger
0
SIX2 protein, human
0
Transcription Factors
0
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
913Subventions
Organisme : NIDDK NIH HHS
ID : U24 DK076169
Pays : United States
Organisme : NCATS NIH HHS
ID : UG3 TR002155
Pays : United States
Organisme : NHGRI NIH HHS
ID : UM1 HG009390
Pays : United States
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