Up-regulation of Rac1 in the bronchial smooth muscle of murine experimental asthma.
Animals
Asthma
/ immunology
Bronchi
/ drug effects
Carbachol
/ pharmacology
Disease Models, Animal
Humans
Male
Mice
Muscle Contraction
/ drug effects
Muscle, Smooth
/ drug effects
Neuropeptides
/ antagonists & inhibitors
Ovalbumin
/ administration & dosage
Pyrones
/ pharmacology
Quinolines
/ pharmacology
Signal Transduction
/ drug effects
Up-Regulation
rac1 GTP-Binding Protein
/ antagonists & inhibitors
Journal
Basic & clinical pharmacology & toxicology
ISSN: 1742-7843
Titre abrégé: Basic Clin Pharmacol Toxicol
Pays: England
ID NLM: 101208422
Informations de publication
Date de publication:
Jul 2019
Jul 2019
Historique:
received:
13
11
2018
accepted:
16
01
2019
pubmed:
31
1
2019
medline:
15
1
2020
entrez:
31
1
2019
Statut:
ppublish
Résumé
There has been considerable research on the involvement of RhoA/Rho kinase signalling in smooth muscle contractions. However, only a few reports have addressed the specific role of Rac1, which is a member of the Rho GTPase superfamily. Therefore, this study investigated the role of Rac1-related pathways in bronchial smooth muscle (BSM) contractions. Bronchial rings isolated from mice were suspended in an organ bath, and the isometric contractions of circular smooth muscles were monitored. The phosphorylation of myosin light chains (MLCs) was analysed by immunoblotting. The Rac1 inhibitor EHT1864 inhibited carbachol (CCh)-induced BSM contractions, although high K
Substances chimiques
EHT 1864
0
Neuropeptides
0
Pyrones
0
Quinolines
0
Rac1 protein, mouse
0
Carbachol
8Y164V895Y
Ovalbumin
9006-59-1
rac1 GTP-Binding Protein
EC 3.6.5.2
Banques de données
GENBANK
['NM_008084.2', 'NM_009007.2']
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
8-15Subventions
Organisme : MEXT-Supported Program for the Strategic Research Foundation at Private Universities
ID : S1411019
Informations de copyright
© 2019 Nordic Association for the Publication of BCPT (former Nordic Pharmacological Society).