Autologous bone marrow-derived cells for venous leg ulcers treatment: a pilot study.


Journal

Cytotherapy
ISSN: 1477-2566
Titre abrégé: Cytotherapy
Pays: England
ID NLM: 100895309

Informations de publication

Date de publication:
02 2019
Historique:
received: 18 10 2018
revised: 30 12 2018
accepted: 08 01 2019
pubmed: 1 2 2019
medline: 25 2 2020
entrez: 1 2 2019
Statut: ppublish

Résumé

Chronic venous leg ulcers (VLUs) are a common problem in clinical practice and available treatments are not satisfactory. The use of adjuvant therapies in combination with lower limb compression may lead to improved healing rates. Chronic wounds are candidates for new strategies in the emergent field of regenerative medicine. Bone marrow-derived cells (BMDCs) contain cells and secrete cytokines known to participate in wound healing. Thus, BMDC therapy seems a logical strategy for the treatment of chronic wounds. Our objective was to evaluate feasibility, safety and initial clinical outcome of autologous BMDC therapy associated with standard treatment in patients with VLUs. We conducted an open-label, single-arm, prospective pilot clinical trial in four patients with six chronic VLUs. The study protocol was approved by the institutional and national review boards and ethics committees. Bone marrow was harvest, processed and then administered by multiple injections into the ulcers. All patients received standard treatment and non-healing characteristics of the VLUs were confirmed at study entry. Ulcer size and wound pain evaluated 12 months after BMDC treatment were significantly reduced (P < 0.05). BMDC treatment was safe and well tolerated in long-term follow-up. Despite the low number of patients studied, our results showed that autologous BMDC treatment could be a useful, feasible and safe procedure to enhance ulcer healing. However, randomized controlled trials with more patients are needed to address this question and translate this approach into clinical practice.

Sections du résumé

BACKGROUND
Chronic venous leg ulcers (VLUs) are a common problem in clinical practice and available treatments are not satisfactory. The use of adjuvant therapies in combination with lower limb compression may lead to improved healing rates. Chronic wounds are candidates for new strategies in the emergent field of regenerative medicine. Bone marrow-derived cells (BMDCs) contain cells and secrete cytokines known to participate in wound healing. Thus, BMDC therapy seems a logical strategy for the treatment of chronic wounds. Our objective was to evaluate feasibility, safety and initial clinical outcome of autologous BMDC therapy associated with standard treatment in patients with VLUs.
METHODS
We conducted an open-label, single-arm, prospective pilot clinical trial in four patients with six chronic VLUs. The study protocol was approved by the institutional and national review boards and ethics committees. Bone marrow was harvest, processed and then administered by multiple injections into the ulcers. All patients received standard treatment and non-healing characteristics of the VLUs were confirmed at study entry.
RESULTS
Ulcer size and wound pain evaluated 12 months after BMDC treatment were significantly reduced (P < 0.05). BMDC treatment was safe and well tolerated in long-term follow-up.
DISCUSSION
Despite the low number of patients studied, our results showed that autologous BMDC treatment could be a useful, feasible and safe procedure to enhance ulcer healing. However, randomized controlled trials with more patients are needed to address this question and translate this approach into clinical practice.

Identifiants

pubmed: 30700393
pii: S1465-3249(19)30002-7
doi: 10.1016/j.jcyt.2019.01.002
pii:
doi:

Types de publication

Clinical Trial Journal Article Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Pagination

189-199

Informations de copyright

Copyright © 2019 International Society for Cell and Gene Therapy. Published by Elsevier Inc. All rights reserved.

Auteurs

Gabriela Otero (G)

Dermatology Department, Hospital de Clínicas Dr Manuel Quintela, Faculty of Medicine, Universidad de la República, Uruguay.

Caroline Agorio (C)

Dermatology Department, Hospital de Clínicas Dr Manuel Quintela, Faculty of Medicine, Universidad de la República, Uruguay.

Alexandra Sujanov (A)

Cell Therapy and Regenerative Medicine Unit, Hospital de Clínicas Dr Manuel Quintela, Faculty of Medicine, Universidad de la República, Uruguay.

Lourdes Echarte (L)

Cell Therapy and Regenerative Medicine Unit, Hospital de Clínicas Dr Manuel Quintela, Faculty of Medicine, Universidad de la República, Uruguay.

Ana Tchekmedyian (A)

Centro de Tratamiento de Enfermedades de la Piel (CETEP), Centro Hospitalario Pereira Rossell, Uruguay.

Monica Montelongo (M)

Transfusion Medicine Department, Hospital de Clínicas Dr Manuel Quintela, Faculty of Medicine, Universidad de la República, Uruguay.

Alba Menyou (A)

Transfusion Medicine Department, Hospital de Clínicas Dr Manuel Quintela, Faculty of Medicine, Universidad de la República, Uruguay.

Andres Rodriguez (A)

Transfusion Medicine Department, Hospital de Clínicas Dr Manuel Quintela, Faculty of Medicine, Universidad de la República, Uruguay.

Lilian Diaz (L)

Hematology Department, Hospital de Clínicas Dr Manuel Quintela, Faculty of Medicine, Universidad de la República, Uruguay.

Ismael Rodriguez (I)

Transfusion Medicine Department, Hospital de Clínicas Dr Manuel Quintela, Faculty of Medicine, Universidad de la República, Uruguay.

Cristina Touriño (C)

Cell Therapy and Regenerative Medicine Unit Medicine Department, Hospital de Clínicas Dr Manuel Quintela, Faculty of Medicine, Universidad de la República, Uruguay. Electronic address: cltourino@gmail.com.

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Classifications MeSH