OVX836 a recombinant nucleoprotein vaccine inducing cellular responses and protective efficacy against multiple influenza A subtypes.


Journal

NPJ vaccines
ISSN: 2059-0105
Titre abrégé: NPJ Vaccines
Pays: England
ID NLM: 101699863

Informations de publication

Date de publication:
2019
Historique:
received: 27 03 2018
accepted: 21 12 2018
entrez: 1 2 2019
pubmed: 1 2 2019
medline: 1 2 2019
Statut: epublish

Résumé

Inactivated influenza vaccines (IIVs) lack broad efficacy. Cellular immunity to a conserved internal antigen, the nucleoprotein (NP), has been correlated to protection against pandemic and seasonal influenza and thus could have the potential to broaden vaccine efficacy. We developed OVX836, a recombinant protein vaccine based on an oligomerized NP, which shows increased uptake by dendritic cells and immunogenicity compared with NP. Intramuscular immunization in mice with OVX836 induced strong NP-specific CD4+ and CD8+ T-cell systemic responses and established CD8+ tissue memory T cells in the lung parenchyma. Strikingly, OVX836 protected mice against viral challenge with three different influenza A subtypes, isolated several decades apart and induced a reduction in viral load. When co-administered with IIV, OVX836 was even more effective in reducing lung viral load.

Identifiants

pubmed: 30701093
doi: 10.1038/s41541-019-0098-4
pii: 98
pmc: PMC6344521
doi:

Types de publication

Journal Article

Langues

eng

Pagination

4

Déclaration de conflit d'intérêts

J.d.C. and F.H. are named as inventors on a family of patents/patent applications “Influenza Nucleoprotein vaccines” (WO 2014/090905, WO 2014/147087), and both are shareholders and employees of OSIVAX. The remaining authors declare no competing interests.

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Auteurs

Judith Del Campo (J)

Osivax, 99, rue de Gerland, 69007 Lyon, France.

Andres Pizzorno (A)

2Virologie et Pathologie Humaine - VirPath Team, Centre International de Recherche en Infectiologie, INSERM U1111, CNRS UMR5308, École Normale Supérieure de Lyon, Université Claude Bernard Lyon 1. Université de Lyon, Lyon, F- 69008 France.

Sophia Djebali (S)

3Immunity and Cytotoxic Lymphocytes, Centre International de Recherche en Infectiologie, INSERM, U1111, Université Claude Bernard Lyon 1, CNRS, UMR5308, École Normale Supérieure de Lyon. Université de Lyon, F-69007 Lyon, France.

Julien Bouley (J)

Osivax, 99, rue de Gerland, 69007 Lyon, France.

Marjorie Haller (M)

Osivax, 99, rue de Gerland, 69007 Lyon, France.

Jimena Pérez-Vargas (J)

Osivax, 99, rue de Gerland, 69007 Lyon, France.
6Present Address: Enveloped Viruses, Vectors and Immunotherapy Team, Centre International de Recherché en Infectiologie (CIRI), INSERM U1111, Université de Lyon, Lyon, France.

Bruno Lina (B)

2Virologie et Pathologie Humaine - VirPath Team, Centre International de Recherche en Infectiologie, INSERM U1111, CNRS UMR5308, École Normale Supérieure de Lyon, Université Claude Bernard Lyon 1. Université de Lyon, Lyon, F- 69008 France.
Hospices Civils de Lyon, Centre National de Référence des Virus Influenza France Sud, Laboratoire de Virologie, Groupement Hospitalier Nord, Lyon, France.

Guy Boivin (G)

5Centre de Recherche en Infectiologie of the Centre Hospitalier Universitaire de Québec and Université Laval, Québec, Canada.

Marie-Eve Hamelin (ME)

5Centre de Recherche en Infectiologie of the Centre Hospitalier Universitaire de Québec and Université Laval, Québec, Canada.

Florence Nicolas (F)

Osivax, 99, rue de Gerland, 69007 Lyon, France.

Alexandre Le Vert (A)

Osivax, 99, rue de Gerland, 69007 Lyon, France.

Yann Leverrier (Y)

3Immunity and Cytotoxic Lymphocytes, Centre International de Recherche en Infectiologie, INSERM, U1111, Université Claude Bernard Lyon 1, CNRS, UMR5308, École Normale Supérieure de Lyon. Université de Lyon, F-69007 Lyon, France.

Manuel Rosa-Calatrava (M)

2Virologie et Pathologie Humaine - VirPath Team, Centre International de Recherche en Infectiologie, INSERM U1111, CNRS UMR5308, École Normale Supérieure de Lyon, Université Claude Bernard Lyon 1. Université de Lyon, Lyon, F- 69008 France.

Jacqueline Marvel (J)

3Immunity and Cytotoxic Lymphocytes, Centre International de Recherche en Infectiologie, INSERM, U1111, Université Claude Bernard Lyon 1, CNRS, UMR5308, École Normale Supérieure de Lyon. Université de Lyon, F-69007 Lyon, France.

Fergal Hill (F)

Osivax, 99, rue de Gerland, 69007 Lyon, France.

Classifications MeSH