Differential responses of myoblasts and myotubes to photobiomodulation are associated with mitochondrial number.
low-level laser therapy
low-level light therapy
mitochondria
myogenesis
photobiomodulation
Journal
Journal of biophotonics
ISSN: 1864-0648
Titre abrégé: J Biophotonics
Pays: Germany
ID NLM: 101318567
Informations de publication
Date de publication:
06 2019
06 2019
Historique:
received:
06
11
2018
revised:
22
01
2019
accepted:
29
01
2019
pubmed:
1
2
2019
medline:
15
7
2020
entrez:
1
2
2019
Statut:
ppublish
Résumé
Photobiomodulation (PBM) is the application of light to promote tissue healing. Current indications suggest PBM induces its beneficial effects in vivo through upregulation of mitochondrial activity. However, how mitochondrial content influences such PBM responses have yet to be evaluated. Hence, the current study assessed the biological response of cells to PBM with varying mitochondrial contents. DNA was isolated from myoblasts and myotubes (differentiated myoblasts), and mitochondrial DNA (mtDNA) was amplified and quantified using a microplate assay. Cells were seeded in 96-wellplates, incubated overnight and subsequently irradiated using a light-emitting diode array (400, 450, 525, 660, 740, 810, 830 and white light, 24 mW/cm Myotubes exhibited mtDNA levels 86% greater than myoblasts (P < 0.001). Irradiation of myotubes at 400, 450 or 810 nm induced 53%, 29% and 47% increases (relative to non-irradiated control) in maximal respiratory rates, respectively (P < 0.001). Conversely, irradiation of myoblasts at 400 or 450 nm had no significant effect on maximal respiratory rates. This study suggests that mitochondrial content may influence cellular responses to PBM and as such explain the variability of PBM responses seen in the literature.
Identifiants
pubmed: 30701682
doi: 10.1002/jbio.201800411
pmc: PMC7065641
doi:
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
e201800411Subventions
Organisme : Medical Research Council
ID : MC_PC_17183
Pays : United Kingdom
Organisme : Engineering and Physical Sciences Research Council
Pays : International
Informations de copyright
© 2019 The Authors. Journal of Biophotonics published by WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.
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