A localized surface plasmon resonance-amplified immunofluorescence biosensor for ultrasensitive and rapid detection of nonstructural protein 1 of Zika virus.
Journal
PloS one
ISSN: 1932-6203
Titre abrégé: PLoS One
Pays: United States
ID NLM: 101285081
Informations de publication
Date de publication:
2019
2019
Historique:
received:
04
12
2018
accepted:
15
01
2019
entrez:
1
2
2019
pubmed:
1
2
2019
medline:
13
11
2019
Statut:
epublish
Résumé
Among the members of flaviviruses, the Zika virus (ZIKV) remains a potent infectious disease agent, with its associated pandemic prompting the World Health Organization (WHO) to declare it a global public health concern. Thus, rapid and accurate diagnosis of the ZIKV is needed. In this study, we report a new immunofluorescence biosensor for the detection of nonstructural protein 1 (NS1) of the ZIKV, which operates using the localized surface plasmon resonance (LSPR) signal from plasmonic gold nanoparticles (AuNPs) to amplify the fluorescence intensity signal of quantum dots (QDs) within an antigen-antibody detection process. The LSPR signal from the AuNPs was used to amplify the fluorescence intensity of the QDs. For ultrasensitive, rapid, and quantitative detection of NS1 of the ZIKV, four different thiol-capped AuNPs were investigated. Our biosensor could detect the ZIKV in a wide concentration range from 10-107 RNA copies/mL, and we found that the limit of detection (LOD) for the ZIKV followed the order Ab-L-cysteine-AuNPs (LOD = 8.2 copies/mL) > Ab-3-mercaptopropionic acid-AuNPs (LOD = 35.0 copies/mL). Immunofluorescence biosensor for NS1 exhibited excellent specificity against other negative control targets and could also detect the ZIKV in human serum.
Identifiants
pubmed: 30703161
doi: 10.1371/journal.pone.0211517
pii: PONE-D-18-34699
pmc: PMC6355018
doi:
Substances chimiques
NS1 protein, zika virus
0
Viral Nonstructural Proteins
0
Gold
7440-57-5
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
e0211517Déclaration de conflit d'intérêts
The authors have declared that no competing interests exist.
Références
Anal Chem. 2016 Jul 19;88(14):7289-94
pubmed: 27306491
Nat Commun. 2018 Apr 11;9(1):1391
pubmed: 29643334
Chem Rev. 2007 Nov;107(11):4797-862
pubmed: 17999554
Nat Struct Mol Biol. 2016 Sep;23(9):865-7
pubmed: 27455458
Virol J. 2013 Oct 22;10:311
pubmed: 24148652
Lancet. 2016 Apr 9;387(10027):1531-1539
pubmed: 26948433
Biosens Bioelectron. 2018 Feb 15;100:85-88
pubmed: 28865242
Nanoscale. 2018 Jul 5;10(25):11841-11849
pubmed: 29881853
Biosens Bioelectron. 2015 Feb 15;64:311-7
pubmed: 25240957
Biosens Bioelectron. 2017 Aug 15;94:513-522
pubmed: 28343104
Euro Surveill. 2016;21(10):30161
pubmed: 26988027
Sensors (Basel). 2017 Oct 13;17(10):
pubmed: 29027923
Sci Rep. 2018 Aug 20;8(1):12438
pubmed: 30127503
Sci Rep. 2017 Mar 20;7:44778
pubmed: 28317856
Trends Biotechnol. 2017 Apr;35(4):308-317
pubmed: 28277248
J Glob Infect Dis. 2016 Jan-Mar;8(1):3-15
pubmed: 27013839
N Engl J Med. 2016 Apr 21;374(16):1552-63
pubmed: 27028561
J Pathol Clin Res. 2017 Dec 04;4(1):19-25
pubmed: 29416874
Biosens Bioelectron. 2017 Jan 15;87:558-565
pubmed: 27611475
Euro Surveill. 2016 Aug 11;21(32):
pubmed: 27542178
Antiviral Res. 2016 Jun;130:69-80
pubmed: 26996139
Biosens Bioelectron. 2017 Mar 15;89(Pt 2):998-1005
pubmed: 27825520
Nat Struct Mol Biol. 2016 May;23(5):456-8
pubmed: 27088990
J Mater Chem B. 2017 Apr 28;5(16):3047-3058
pubmed: 32263996
Nat Rev Microbiol. 2016 Nov;14(11):707-715
pubmed: 27573577
Front Microbiol. 2016 Oct 24;7:1685
pubmed: 27822207
Euro Surveill. 2014 Apr 10;19(14):
pubmed: 24739982
J Virol. 1998 Jan;72(1):73-83
pubmed: 9420202