Intraoperative loading of calcium phosphate-coated implants with gentamicin prevents experimental Staphylococcus aureus infection in vivo.
Alloys
/ pharmacology
Animals
Bone Screws
Calcium Phosphates
/ pharmacology
Coated Materials, Biocompatible
/ pharmacology
Female
Gentamicins
/ pharmacology
Intraoperative Care
Niobium
/ pharmacology
Rats
Rats, Wistar
Staphylococcal Infections
/ prevention & control
Staphylococcus aureus
/ growth & development
Tibia
/ metabolism
Journal
PloS one
ISSN: 1932-6203
Titre abrégé: PLoS One
Pays: United States
ID NLM: 101285081
Informations de publication
Date de publication:
2019
2019
Historique:
received:
11
10
2018
accepted:
21
12
2018
entrez:
2
2
2019
pubmed:
2
2
2019
medline:
30
10
2019
Statut:
epublish
Résumé
Orthopedic device-related infection (ODRI) is a potentially devastating complication arising from the colonization of the device with bacteria, such as Staphylococcus aureus. The aim of this study was to determine if intraoperative loading of a clinically approved calcium phosphate (CaP) coating with gentamicin can protect from ODRI in vivo. First, CaP-coated titanium aluminium niobium (TAN) discs were used to investigate the adsorption and release kinetics of gentamicin in vitro. Gentamicin loading and subsequent release from the coating were both rapid, with maximum loading occurring following one second of immersion, and >95% gentamicin released within 15 min in aqueous solution, respectively. Second, efficacy of the gentamicin-loaded CaP coating for preventing ODRI in vivo was investigated using a CaP-coated unicortical TAN screw implanted into the proximal tibia of skeletally mature female Wistar rats, following inoculation of the implant site with S. aureus. Gentamicin-loading prevented ODRI in 7/8 animals, whereas 9/9 of the non-gentamicin treated animals were infected after 7 days. In conclusion, gentamicin can be rapidly and simply loaded onto, and released from, CaP-based implant coatings, and this is an effective strategy for preventing peri-operative S. aureus-induced ODRI in vivo.
Identifiants
pubmed: 30707699
doi: 10.1371/journal.pone.0210402
pii: PONE-D-18-29564
pmc: PMC6358082
doi:
Substances chimiques
Alloys
0
Calcium Phosphates
0
Coated Materials, Biocompatible
0
Gentamicins
0
titanium aluminum alloy
0
Niobium
05175J654G
calcium phosphate
97Z1WI3NDX
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
e0210402Déclaration de conflit d'intérêts
The authors have declared that no competing interests exist.
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