Minimal nanodisc without exogenous lipids for stabilizing membrane proteins in detergent-free buffer.
CryoEM
Lipids
Membrane protein
Nanodisc
Journal
Biochimica et biophysica acta. Biomembranes
ISSN: 1879-2642
Titre abrégé: Biochim Biophys Acta Biomembr
Pays: Netherlands
ID NLM: 101731713
Informations de publication
Date de publication:
01 04 2019
01 04 2019
Historique:
received:
21
09
2018
revised:
17
12
2018
accepted:
24
01
2019
pubmed:
2
2
2019
medline:
27
11
2019
entrez:
2
2
2019
Statut:
ppublish
Résumé
Membrane protein stabilization after detergent solubilization presents drawbacks for structural and biophysical studies, in particular that of a reduced stability in detergent micelles. Therefore, alternative methods are required for efficient stabilization. Lipid nanodisc made with the membrane scaffold protein MSP is a valuable system but requires a fine optimization of the lipid to protein ratio. We present here the use of the scaffold protein MSP without added lipids as a minimal system to stabilize membrane proteins. We show that this method is applicable to α-helical and β-strands transmembrane proteins. This method allowed cryo-electron microscopy structural study of the bacterial transporter MexB. A protein quantification indicates that MexB is stabilized by two MSP proteins. This simplified and efficient method proposes a new advance in harnessing the MSP potential to stabilize membrane proteins.
Identifiants
pubmed: 30707889
pii: S0005-2736(19)30024-0
doi: 10.1016/j.bbamem.2019.01.013
pii:
doi:
Substances chimiques
Bacterial Outer Membrane Proteins
0
Buffers
0
Membrane Lipids
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
852-860Informations de copyright
Copyright © 2019 Elsevier B.V. All rights reserved.