A new application of Trichoderma asperellum as an anopheline larvicide for eco friendly management in medical science.
Journal
Scientific reports
ISSN: 2045-2322
Titre abrégé: Sci Rep
Pays: England
ID NLM: 101563288
Informations de publication
Date de publication:
01 02 2019
01 02 2019
Historique:
received:
21
05
2018
accepted:
30
11
2018
entrez:
3
2
2019
pubmed:
3
2
2019
medline:
21
10
2020
Statut:
epublish
Résumé
Microfungal applications are increasing daily in the medical science. Several species of Trichoderma are widely used in agricultural fields as biological control and plant growth promoting agents. The application of Trichoderma asperellum as an entomopathogenic fungus against the Anopheles mosquito, a vector of malaria, is a novel control approach. Controlling malaria with eco-friendly management practices is an urgent need. We isolated three T. asperellum from different natural sources using serial dilution and mosquito baiting techniques. The fungi were identified on the basis of phenotypical and molecular characteristics. The fungi were grown in different natural media to examine spore production ability and the fungal spore suspensions were applied to the anopheline larvae to determine their larvicidal activity in vitro. We investigated the efficacy of crude ME (methanolic extract) and different methanolic fractions (MFs) of the fungal extracts against anopheline larvae. Methanolic Fraction 8 (MF8) exhibited the strongest larvicidal activity. A GC-MS analysis of MF8 and a Chemolibrary search were performed to identify the active agents in the fungal extracts. Among the three isolates of T. asperellum, the TaspSKGN2 isolate showed the lowest LD
Identifiants
pubmed: 30710113
doi: 10.1038/s41598-018-37108-2
pii: 10.1038/s41598-018-37108-2
pmc: PMC6358612
doi:
Substances chimiques
Insecticides
0
Plant Extracts
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
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