MicroRNA hsa-miR-29a-3p is a plasma biomarker for the differential diagnosis and monitoring of tuberculosis.
Adolescent
Adult
Aged
Antitubercular Agents
/ administration & dosage
Biomarkers
/ blood
CD4 Lymphocyte Count
Case-Control Studies
Coinfection
/ diagnosis
Diagnosis, Differential
Drug Administration Schedule
Female
Gene Expression Profiling
/ methods
HIV Infections
/ complications
Humans
Latent Tuberculosis
/ complications
Male
MicroRNAs
/ blood
Middle Aged
Tuberculosis
/ complications
Up-Regulation
Young Adult
Biomarker
Diagnosis
Pediatric/extra-pulmonary tuberculosis
Performance
microRNA
Journal
Tuberculosis (Edinburgh, Scotland)
ISSN: 1873-281X
Titre abrégé: Tuberculosis (Edinb)
Pays: Scotland
ID NLM: 100971555
Informations de publication
Date de publication:
01 2019
01 2019
Historique:
received:
07
07
2018
revised:
28
11
2018
accepted:
02
12
2018
entrez:
4
2
2019
pubmed:
4
2
2019
medline:
2
7
2019
Statut:
ppublish
Résumé
The diagnosis of tuberculosis (TB) continues to pose substantial public health problems. The quest for diagnostic biomarkers for TB is therefore primordial. This study aimed to evaluate the diagnostic and anti-TB treatment monitoring potentials of some selected miRNAs. Quantitative real time polymerase chain reaction and Receiver operating characteristics were used to estimate the ability of miRNAs to discriminate between healthy controls (HEC), latent (LTB) and active TB (ATB). The study showed that: hsa-miR-29a-3p, hsa-miR-155-5p and hsa-miR-361-5p were significantly upregulated in ATB compared to HEC while hsa-miR-29a-3p, and hsa-miR-361-5p were also significantly up-regulated in ATB compared to LTB (all P ≤ 0.05). MiR-29a-3p showed a good (81.37%) distinguishing performance in discriminating ATB from HEC and a good (84.35%) diagnostic performance in discriminating ATB from LTB. The performance of miR-29a-3p present in the blood in discriminating active TB from latent TB and healthy controls indicates it may be a useful biomarker for diagnosis of TB. Because this miRNA is found in blood (plasma) which is easy to collect compared to sputum it could be used in pediatric and extra-pulmonary TB cases.
Identifiants
pubmed: 30711160
pii: S1472-9792(18)30277-4
doi: 10.1016/j.tube.2018.12.001
pii:
doi:
Substances chimiques
Antitubercular Agents
0
Biomarkers
0
MIRN29a microRNA, human
0
MicroRNAs
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
69-76Informations de copyright
Copyright © 2018. Published by Elsevier Ltd.