A methodology study on chemical and molecular structure imaging in modified forage leaf tissue with cutting-edge synchrotron-powered technology (SR-IMS) as a potential research tool.


Journal

Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy
ISSN: 1873-3557
Titre abrégé: Spectrochim Acta A Mol Biomol Spectrosc
Pays: England
ID NLM: 9602533

Informations de publication

Date de publication:
15 Apr 2019
Historique:
received: 23 10 2018
revised: 26 12 2018
accepted: 20 01 2019
pubmed: 4 2 2019
medline: 22 3 2019
entrez: 4 2 2019
Statut: ppublish

Résumé

To date there is no any study on imaging molecular chemistry and chemical structure of biotech-modified plant tissue on a molecular basis. The objective of this methodology study was to apply a non-invasive and non-destructive synchrotron powered technology - SR-IMS to image molecular chemistry of the modified forage leaf tissue. The infrared molecular vibrational microspectroscopy powered with synchrotron light at Advanced Light Source (ALS, Lawrence Berkeley National Lab, Berkeley, California, Dept. of Energy, USA) were applied. The synchrotron beamline time was arranged by National Synchrotron Light Source (Scientist Dr. Lisa Miller, Brookhaven National Lab, Dept. of Energy, USA). The various molecular functional groups in the forage tissue included CH symmetric and asymmetric regions, amides I and II regions, structure and non-structure CHO regions, carbonyl ester region with peak areas at ca. 3644-3000 cm

Identifiants

pubmed: 30711903
pii: S1386-1425(19)30079-4
doi: 10.1016/j.saa.2019.01.064
pii:
doi:

Substances chimiques

Amides 0

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Pagination

330-336

Informations de copyright

Copyright © 2019. Published by Elsevier B.V.

Auteurs

Peiqiang Yu (P)

Henan University of Animal Husbandry and Economy, Zhengzhou, China; College of Agriculture and Bioresources, University of Saskatchewan, 51 Campus Drive, Saskatoon, SK S7N 5A8, Canada. Electronic address: peiqiang.yu@usask.ca.

Yaogeng Lei (Y)

Henan University of Animal Husbandry and Economy, Zhengzhou, China; College of Agriculture and Bioresources, University of Saskatchewan, 51 Campus Drive, Saskatoon, SK S7N 5A8, Canada.

Huafeng Hu (H)

Henan University of Animal Husbandry and Economy, Zhengzhou, China; College of Agriculture and Bioresources, University of Saskatchewan, 51 Campus Drive, Saskatoon, SK S7N 5A8, Canada.

Hongyu Deng (H)

Henan University of Animal Husbandry and Economy, Zhengzhou, China; College of Agriculture and Bioresources, University of Saskatchewan, 51 Campus Drive, Saskatoon, SK S7N 5A8, Canada.

Weixian Zhang (W)

Henan University of Animal Husbandry and Economy, Zhengzhou, China; College of Agriculture and Bioresources, University of Saskatchewan, 51 Campus Drive, Saskatoon, SK S7N 5A8, Canada. Electronic address: zhangwx126@126.com.

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Classifications MeSH