ELTA: Enzymatic Labeling of Terminal ADP-Ribose.
ADP-ribose
ADP-ribosylated protein
ADP-ribosylation
ADP-ribosyltransferase
enzymatic labeling
mono(ADP-ribosyl)ated protein
oligoadenylate synthetase
poly(ADP-ribose)
poly(ADP-ribose) polymerase
poly(ADP-ribosyl)ated protein
Journal
Molecular cell
ISSN: 1097-4164
Titre abrégé: Mol Cell
Pays: United States
ID NLM: 9802571
Informations de publication
Date de publication:
21 02 2019
21 02 2019
Historique:
received:
13
06
2018
revised:
22
10
2018
accepted:
21
12
2018
pubmed:
5
2
2019
medline:
25
6
2019
entrez:
5
2
2019
Statut:
ppublish
Résumé
ADP-ribosylation refers to the addition of one or more ADP-ribose groups onto proteins. The attached ADP-ribose monomers or polymers, commonly known as poly(ADP-ribose) (PAR), modulate the activities of the modified substrates or their binding affinities to other proteins. However, progress in this area is hindered by a lack of tools to investigate this protein modification. Here, we describe a new method named ELTA (enzymatic labeling of terminal ADP-ribose) for labeling free or protein-conjugated ADP-ribose monomers and polymers at their 2'-OH termini using the enzyme OAS1 and dATP. When coupled with various dATP analogs (e.g., radioactive, fluorescent, affinity tags), ELTA can be used to explore PAR biology with techniques routinely used to investigate DNA or RNA function. We demonstrate that ELTA enables the biophysical measurements of protein binding to PAR of a defined length, detection of PAR length from proteins and cells, and enrichment of sub-femtomole amounts of ADP-ribosylated peptides from cell lysates.
Identifiants
pubmed: 30712989
pii: S1097-2765(18)31098-0
doi: 10.1016/j.molcel.2018.12.022
pmc: PMC6629254
mid: NIHMS1517746
pii:
doi:
Substances chimiques
Deoxyadenine Nucleotides
0
Adenosine Diphosphate Ribose
20762-30-5
2'-deoxy-5'-adenosine monophosphate
653-63-4
RNF146 protein, human
EC 2.3.2.27
Ubiquitin-Protein Ligases
EC 2.3.2.27
Poly(ADP-ribose) Polymerases
EC 2.4.2.30
OAS1 protein, human
EC 2.7.7.-
2',5'-Oligoadenylate Synthetase
EC 2.7.7.84
2'-deoxyadenosine triphosphate
K8KCC8SH6N
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
845-856.e5Subventions
Organisme : NIAMS NIH HHS
ID : R01 AR065459
Pays : United States
Organisme : NCI NIH HHS
ID : T32 CA009110
Pays : United States
Organisme : NIH HHS
ID : S10 OD021502
Pays : United States
Organisme : NCI NIH HHS
ID : P30 CA045508
Pays : United States
Organisme : NIGMS NIH HHS
ID : T32 GM080189
Pays : United States
Organisme : NIGMS NIH HHS
ID : R01 GM104135
Pays : United States
Informations de copyright
Copyright © 2018 Elsevier Inc. All rights reserved.
Références
Biochim Biophys Acta. 1999 Aug 5;1428(2-3):137-46
pubmed: 10434031
Cell Mol Life Sci. 2000 Oct;57(11):1593-612
pubmed: 11092454
J Cell Sci. 2001 Feb;114(Pt 3):459-60
pubmed: 11171313
Nucleic Acids Res. 2007;35(21):e143
pubmed: 17991682
Curr Protoc Protein Sci. 2008 Feb;Chapter 5:Unit 5.20
pubmed: 18429060
Mol Cell. 2008 Oct 10;32(1):57-69
pubmed: 18851833
Trends Biochem Sci. 2010 Apr;35(4):208-19
pubmed: 20106667
Nat Rev Cancer. 2010 Apr;10(4):293-301
pubmed: 20200537
Chem Soc Rev. 2010 Apr;39(4):1272-9
pubmed: 20349533
Biochemistry. 2010 Aug 24;49(33):7119-30
pubmed: 20669926
Anal Biochem. 1990 Dec;191(2):309-13
pubmed: 2085177
J Am Chem Soc. 2010 Nov 17;132(45):15878-80
pubmed: 20968292
Nat Commun. 2010 Oct 19;1:100
pubmed: 20981028
J Interferon Cytokine Res. 2011 Jan;31(1):41-7
pubmed: 21142819
Nat Cell Biol. 2011 May;13(5):623-9
pubmed: 21478859
Nature. 2011 Sep 04;477(7366):616-20
pubmed: 21892188
Genes Dev. 2012 Feb 1;26(3):235-40
pubmed: 22267412
Int J Mass Spectrom. 2012 Feb 15;312:114-121
pubmed: 22563295
Anal Biochem. 2012 Sep 15;428(2):126-36
pubmed: 22743307
Nature. 1979 Dec 13;282(5740):740-1
pubmed: 229416
ACS Chem Biol. 2013 Jan 18;8(1):179-88
pubmed: 23082994
Proc Natl Acad Sci U S A. 2013 Jan 29;110(5):1652-7
pubmed: 23319625
Structure. 2013 Mar 5;21(3):462-75
pubmed: 23473667
ACS Chem Biol. 2013 Jul 19;8(7):1567-75
pubmed: 23631432
FEBS J. 2013 Aug;280(15):3491-507
pubmed: 23711178
Nat Rev Mol Cell Biol. 2013 Jul;14(7):443-51
pubmed: 23736681
Nat Methods. 2013 Oct;10(10):981-4
pubmed: 23955771
Nat Commun. 2013;4:2993
pubmed: 24356582
J Am Chem Soc. 2014 Apr 9;136(14):5201-4
pubmed: 24641686
Proc Natl Acad Sci U S A. 2014 May 20;111(20):7278-83
pubmed: 24799691
J Cell Biol. 2014 Jun 9;205(5):613-9
pubmed: 24914234
J Proteome Res. 2014 Aug 1;13(8):3510-22
pubmed: 24920161
Nat Rev Immunol. 2014 Aug;14(8):521-8
pubmed: 25033909
J Virol. 2014 Dec;88(24):14222-31
pubmed: 25275129
J Am Chem Soc. 2015 Mar 18;137(10):3558-64
pubmed: 25706250
Annu Rev Biochem. 2015;84:227-63
pubmed: 25747399
Biochem J. 2015 Jun 1;468(2):293-301
pubmed: 25789582
Chem Biol. 2015 Apr 23;22(4):446-452
pubmed: 25865309
Structure. 2015 May 5;23(5):851-862
pubmed: 25892109
DNA Repair (Amst). 2015 Aug;32:10-16
pubmed: 25963443
Mol Cell. 2015 Jun 18;58(6):911-24
pubmed: 26091340
Mol Cell. 2015 Jun 18;58(6):959-69
pubmed: 26091344
BMC Biochem. 2015 Jun 26;16:15
pubmed: 26113370
Sci Rep. 2015 Dec 16;5:18271
pubmed: 26669448
Mol Cell. 2016 May 5;62(3):432-442
pubmed: 27067600
Science. 2016 Jul 1;353(6294):45-50
pubmed: 27256882
Angew Chem Int Ed Engl. 2016 Aug 26;55(36):10634-8
pubmed: 27464500
Nucleic Acids Res. 2017 Jan 4;45(D1):D204-D209
pubmed: 27507885
Genes Dev. 2016 Aug 15;30(16):1796-810
pubmed: 27601529
Nat Commun. 2016 Sep 30;7:12917
pubmed: 27686526
ACS Chem Biol. 2016 Nov 18;11(11):3179-3190
pubmed: 27689388
Nat Chem Biol. 2016 Dec;12(12):998-1000
pubmed: 27723750
Nucleic Acids Res. 2017 Apr 7;45(6):3528-3536
pubmed: 27903888
Anal Chem. 2017 Feb 7;89(3):1523-1530
pubmed: 28035797
Nat Chem Biol. 2017 Mar;13(3):302-308
pubmed: 28092360
Proc Natl Acad Sci U S A. 2017 Feb 14;114(7):1666-1671
pubmed: 28143925
Genes Dev. 2017 Jan 15;31(2):101-126
pubmed: 28202539
Br J Pharmacol. 2018 Jan;175(2):192-222
pubmed: 28213892
Science. 2017 Mar 17;355(6330):1152-1158
pubmed: 28302823
FEBS J. 2017 Sep;284(18):2932-2946
pubmed: 28383827
Biochemistry. 2017 Dec 5;56(48):6305-6316
pubmed: 29053245
Cell Rep. 2017 Nov 21;21(8):2326-2337
pubmed: 29166620
Chem Rev. 2018 Feb 14;118(3):1092-1136
pubmed: 29172462
Biochemistry. 2018 Jan 30;57(4):429-440
pubmed: 29327913
Nat Chem Biol. 2018 Feb 14;14(3):236-243
pubmed: 29443986
Org Lett. 2018 Jul 6;20(13):4140-4143
pubmed: 29947522
EMBO Rep. 2018 Aug;19(8):
pubmed: 29954836
Cell Rep. 2018 Aug 28;24(9):2493-2505.e4
pubmed: 30157440
Biochemistry. 1987 Jun 2;26(11):3218-24
pubmed: 3038179
Anal Biochem. 1988 Oct;174(1):239-50
pubmed: 3218735
Nucleic Acids Res. 1980 Jul 25;8(14):3073-85
pubmed: 6160465
Biochemistry. 1984 Jul 31;23(16):3771-7
pubmed: 6206890
Biochem Biophys Res Commun. 1981 May 29;100(2):847-56
pubmed: 6268077
Eur J Biochem. 1982 Mar 1;122(3):601-8
pubmed: 6277637
Proc Natl Acad Sci U S A. 1980 Aug;77(8):4618-22
pubmed: 6933509
J Biol Chem. 1982 Jun 10;257(11):6217-23
pubmed: 7042708
Anal Biochem. 1995 May 1;227(1):1-13
pubmed: 7668367
Biochim Biophys Acta. 1994 Oct 18;1219(2):342-50
pubmed: 7918631
Anal Biochem. 1995 Jul 1;228(2):245-51
pubmed: 8572302
Methods. 1998 Jul;15(3):233-42
pubmed: 9735308