Cost-Effective HPLC-UV Method for Quantification of Vitamin D
LC
dried blood spot
stability study
vitamin D estimation
Journal
Chemical & pharmaceutical bulletin
ISSN: 1347-5223
Titre abrégé: Chem Pharm Bull (Tokyo)
Pays: Japan
ID NLM: 0377775
Informations de publication
Date de publication:
2019
2019
Historique:
entrez:
5
2
2019
pubmed:
5
2
2019
medline:
29
9
2019
Statut:
ppublish
Résumé
25-Hydroxyvitamin D (25-(OH)D) deficiency is recently been described as one of the multiple factors responsible for pediatric seizures. 25-Hydroxyvitamin D3 and 25-Hydroxyvitamin D2 are the well-known markers to determine Vitamin D status. In this work we report the development of a sensitive and cost effective HPLC technique for the quantification of the vitamin D metabolites from dried blood spot samples (DBS). The metabolites were extracted using acetonitrile-methanol-0.1% formic acid (60 : 20 : 20 (v/v)) and analyzed on an Acclaim C
Identifiants
pubmed: 30713278
doi: 10.1248/cpb.c18-00542
doi:
Substances chimiques
Biomarkers
0
25-Hydroxyvitamin D 2
21343-40-8
Calcifediol
P6YZ13C99Q
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM