Alterations of RNA Metabolism by Proteomic Analysis of Breast Cancer Cells Exposed to Marycin: A New Optically Active Porphyrin.
Apoptosis
/ drug effects
Breast Neoplasms
/ metabolism
Cell Cycle Checkpoints
/ drug effects
Cell Line, Tumor
Chromatography, High Pressure Liquid
Female
Gene Expression Regulation, Neoplastic
/ drug effects
Hematoporphyrins
/ chemistry
Humans
Porphyrins
/ chemistry
Proteomics
/ methods
RNA
/ metabolism
RNA Splicing
/ drug effects
Reactive Oxygen Species
/ metabolism
Tandem Mass Spectrometry
LC-MS/MS
MDA-MB-231 breast
cancer cells
Marycin
RNA metabolism
RNA splicing
anti-proliferative effect
peroxisomes
porphyrin.
Journal
Current molecular pharmacology
ISSN: 1874-4702
Titre abrégé: Curr Mol Pharmacol
Pays: United Arab Emirates
ID NLM: 101467997
Informations de publication
Date de publication:
2019
2019
Historique:
received:
05
05
2018
revised:
11
01
2019
accepted:
22
01
2019
pubmed:
5
2
2019
medline:
17
8
2019
entrez:
5
2
2019
Statut:
ppublish
Résumé
Marycin is a porphyrin-type compound synthetically modified to spontaneously release fluorescence. This study is aimed at understanding possible mechanisms that could account for the antiproliferative effects observed in marycin. A proteomic approach was used to identify molecular effects. The proteome of proliferating MDA-MB-231 breast cancer cells was compared with that of marycin-treated cells. Label-free proteomic analysis by liquid chromatography coupled with tandem mass spectrometry (LC-MS/MS) was used to reveal changes in protein expression and fluorescence microscopy and flow cytometry were used to detect subcellular organelle dysfunctions. The bioinformatic analysis indicated an enhancement of the expression of proteins remodeling RNA splicing and more in general, of RNA metabolism. Marycin did not localize into the mitochondria and did not produce a dramatic increase of ROS levels in MDA-MB-231 cells. Marycin stained organelles probably peroxisomes. The results could support the possibility that the peroxisomes are involved in cell response to marycin.
Identifiants
pubmed: 30714537
pii: CMP-EPUB-96320
doi: 10.2174/1874467212666190204102112
doi:
Substances chimiques
Hematoporphyrins
0
Porphyrins
0
Reactive Oxygen Species
0
marycin
111775-32-7
RNA
63231-63-0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Pagination
147-159Informations de copyright
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