Gardnerella vaginalis and Prevotella bivia Trigger Distinct and Overlapping Phenotypes in a Mouse Model of Bacterial Vaginosis.
Animals
Coinfection
/ microbiology
Disease Models, Animal
Female
Gardnerella vaginalis
/ genetics
Humans
Mice
Mice, Inbred C57BL
Microbiota
Neuraminidase
/ analysis
Phenotype
Prevotella
/ genetics
RNA, Bacterial
/ genetics
RNA, Ribosomal, 16S
/ genetics
Reverse Transcriptase Polymerase Chain Reaction
Vagina
/ microbiology
Vaginosis, Bacterial
/ microbiology
coinfection
exfoliation
sialidase
vagina
Journal
The Journal of infectious diseases
ISSN: 1537-6613
Titre abrégé: J Infect Dis
Pays: United States
ID NLM: 0413675
Informations de publication
Date de publication:
30 08 2019
30 08 2019
Historique:
received:
16
08
2018
accepted:
14
01
2019
pubmed:
5
2
2019
medline:
28
5
2020
entrez:
5
2
2019
Statut:
ppublish
Résumé
Bacterial vaginosis (BV) is a common imbalance of the vaginal microbiota characterized by overgrowth of diverse Actinobacteria, Firmicutes, and Gram-negative anaerobes. Women with BV are at increased risk of secondary reproductive tract infections and adverse pregnancy outcomes. However, which specific bacteria cause clinical features of BV is unclear. We previously demonstrated that Gardnerella vaginalis could elicit many BV features in mice. In this study, we established a BV model in which we coinfected mice with G. vaginalis and another species commonly found in women with BV: Prevotella bivia. This coinfection model recapitulates several aspects of human BV, including vaginal sialidase activity (a diagnostic BV feature independently associated with adverse outcomes), epithelial exfoliation, and ascending infection. It is notable that G. vaginalis facilitated uterine infection by P. bivia. Taken together, our model provides a framework for advancing our understanding of the role of individual or combinations of BV-associated bacteria in BV pathogenesis.
Sections du résumé
BACKGROUND
Bacterial vaginosis (BV) is a common imbalance of the vaginal microbiota characterized by overgrowth of diverse Actinobacteria, Firmicutes, and Gram-negative anaerobes. Women with BV are at increased risk of secondary reproductive tract infections and adverse pregnancy outcomes. However, which specific bacteria cause clinical features of BV is unclear.
METHODS
We previously demonstrated that Gardnerella vaginalis could elicit many BV features in mice. In this study, we established a BV model in which we coinfected mice with G. vaginalis and another species commonly found in women with BV: Prevotella bivia.
RESULTS
This coinfection model recapitulates several aspects of human BV, including vaginal sialidase activity (a diagnostic BV feature independently associated with adverse outcomes), epithelial exfoliation, and ascending infection. It is notable that G. vaginalis facilitated uterine infection by P. bivia.
CONCLUSIONS
Taken together, our model provides a framework for advancing our understanding of the role of individual or combinations of BV-associated bacteria in BV pathogenesis.
Identifiants
pubmed: 30715405
pii: 5305674
doi: 10.1093/infdis/jiy704
pmc: PMC6736442
doi:
Substances chimiques
RNA, Bacterial
0
RNA, Ribosomal, 16S
0
Neuraminidase
EC 3.2.1.18
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
1099-1108Subventions
Organisme : NCRR NIH HHS
ID : C06 RR015502
Pays : United States
Organisme : NIDDK NIH HHS
ID : K01 DK110225
Pays : United States
Organisme : NIAID NIH HHS
ID : R01 AI114635
Pays : United States
Organisme : NIAID NIH HHS
ID : R01 AI127554
Pays : United States
Commentaires et corrections
Type : CommentIn
Informations de copyright
© The Author(s) 2019. Published by Oxford University Press for the Infectious Diseases Society of America. All rights reserved. For permissions, e-mail: journals.permissions@oup.com.
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