Feather
HPGDS
MITF
Melanogenesis
Single-cell gene expression
Speciation genetics
Journal
The Journal of experimental biology
ISSN: 1477-9145
Titre abrégé: J Exp Biol
Pays: England
ID NLM: 0243705
Informations de publication
Date de publication:
08 03 2019
08 03 2019
Historique:
received:
12
10
2018
accepted:
31
01
2019
pubmed:
6
2
2019
medline:
19
5
2020
entrez:
6
2
2019
Statut:
epublish
Résumé
Functional validation of candidate genes involved in adaptation and speciation remains challenging. Here, we exemplify the utility of a method quantifying individual mRNA transcripts in revealing the molecular basis of divergence in feather pigment synthesis during early-stage speciation in crows. Using a padlock probe assay combined with rolling circle amplification, we quantified cell-type-specific gene expression in the histological context of growing feather follicles. Expression of Tyrosinase Related Protein 1 (
Identifiants
pubmed: 30718374
pii: jeb.194431
doi: 10.1242/jeb.194431
pmc: PMC6650291
mid: EMS83767
pii:
doi:
Substances chimiques
Pigments, Biological
0
RNA, Messenger
0
Banques de données
figshare
['10.6084/m9.figshare.7635791']
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Subventions
Organisme : European Research Council
ID : 336536
Pays : International
Informations de copyright
© 2019. Published by The Company of Biologists Ltd.
Déclaration de conflit d'intérêts
Competing interestsThe authors declare no competing or financial interests.
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