Measles vector as a multigene delivery platform facilitating iPSC reprogramming.
Journal
Gene therapy
ISSN: 1476-5462
Titre abrégé: Gene Ther
Pays: England
ID NLM: 9421525
Informations de publication
Date de publication:
05 2019
05 2019
Historique:
received:
26
09
2018
accepted:
09
01
2019
revised:
07
12
2018
pubmed:
6
2
2019
medline:
19
12
2019
entrez:
6
2
2019
Statut:
ppublish
Résumé
Induced pluripotent stem cells (iPSCs) provide a unique platform for individualized cell therapy approaches. Currently, episomal DNA, mRNA, and Sendai virus-based RNA reprogramming systems are widely used to generate iPSCs. However, they all rely on the use of multiple (three to six) components (vectors/plasmids/mRNAs) leading to the production of partially reprogrammed cells, reducing the efficiency of the systems. We produced a one-cycle measles virus (MV) vector by substituting the viral attachment protein gene with the green fluorescent protein (GFP) gene. Here, we present a highly efficient multi-transgene delivery system based on a vaccine strain of MV, a non-integrating RNA virus that has a long-standing safety record in humans. Introduction of the four reprogramming factors OCT4, SOX2, KLF4, and cMYC via a single, "one-cycle" MV vector efficiently reprogrammed human somatic cells into iPSCs, whereas MV vector genomes are rapidly eliminated in derived iPSCs. Our MV vector system offers a new reprogramming platform for genomic modification-free iPSCs amenable for clinical translation.
Identifiants
pubmed: 30718755
doi: 10.1038/s41434-019-0058-7
pii: 10.1038/s41434-019-0058-7
pmc: PMC8228481
mid: NIHMS1708522
doi:
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
151-164Subventions
Organisme : NIAID NIH HHS
ID : R21 AI114833
Pays : United States
Organisme : NIAID NIH HHS
ID : R21 AI105233
Pays : United States
Organisme : NCI NIH HHS
ID : P30 CA015083
Pays : United States
Organisme : U.S. Department of Health & Human Services | NIH | National Institute of Allergy and Infectious Diseases (NIAID)
ID : R21AI105233
Pays : International
Organisme : NCATS NIH HHS
ID : UL1 TR002377
Pays : United States
Organisme : U.S. Department of Health & Human Services | NIH | National Center for Advancing Translational Sciences (NCATS)
ID : UL1TR002377
Pays : International
Organisme : NCATS NIH HHS
ID : UL1 TR000135
Pays : United States
Références
Cell. 2006 Aug 25;126(4):663-76
pubmed: 16904174
J Virol. 1999 Nov;73(11):9568-75
pubmed: 10516065
Curr Top Microbiol Immunol. 2009;329:129-62
pubmed: 19198565
Nat Rev Drug Discov. 2017 Feb;16(2):115-130
pubmed: 27980341
Nature. 2011 Mar 3;471(7336):46-7
pubmed: 21368819
Proc Jpn Acad Ser B Phys Biol Sci. 2009;85(8):348-62
pubmed: 19838014
Stem Cells Transl Med. 2016 May;5(5):694-702
pubmed: 26987352
J Gene Med. 2003 Jul;5(7):543-53
pubmed: 12825193
Science. 2009 May 8;324(5928):797-801
pubmed: 19325077
PLoS One. 2014 Dec 05;9(12):e113052
pubmed: 25479600
Regen Med. 2016 Jan;11(1):105-32
pubmed: 26679838
Nat Rev Drug Discov. 2015 Oct;14(10):681-92
pubmed: 26391880
J Virol. 2001 Jan;75(2):910-20
pubmed: 11134304
EMBO J. 1987 Mar;6(3):681-8
pubmed: 3582370
J Virol. 2007 Oct;81(19):10597-605
pubmed: 17634218
J Infect Dis. 2003 May 15;187 Suppl 1:S291-8
pubmed: 12721928
Nat Biotechnol. 2015 Jan;33(1):58-63
pubmed: 25437882
Mol Ther Methods Clin Dev. 2016 Aug 24;3:16057
pubmed: 27606350
Expert Opin Biol Ther. 2017 Mar;17(3):353-363
pubmed: 28129716
Nat Methods. 2009 Nov;6(11):805-8
pubmed: 19838168
N Engl J Med. 2017 Mar 16;376(11):1038-1046
pubmed: 28296613
J Virol. 2000 Jul;74(14):6564-9
pubmed: 10864670
J Virol. 2009 Jan;83(2):961-8
pubmed: 19004947
J Virol. 2001 Jan;75(2):921-33
pubmed: 11134305
Nat Rev Mol Cell Biol. 2016 Mar;17(3):194-200
pubmed: 26908143
Cell Stem Cell. 2010 Nov 5;7(5):618-30
pubmed: 20888316
Science. 2017 Mar 17;355(6330):1109-1110
pubmed: 28302802
Cell. 2007 Nov 30;131(5):861-72
pubmed: 18035408
J Virol. 2006 May;80(9):4242-8
pubmed: 16611883
Mol Ther. 2013 Jan;21(1):228-39
pubmed: 23183535
Nat Methods. 2011 May;8(5):409-12
pubmed: 21460823
Stem Cells Transl Med. 2012 Jun;1(6):451-61
pubmed: 23197849
Virology. 2007 Mar 30;360(1):72-83
pubmed: 17112561
Nat Biotechnol. 2015 Sep;33(9):890-1
pubmed: 26348942
Proc Natl Acad Sci U S A. 2011 Aug 23;108(34):14234-9
pubmed: 21821793
Science. 2007 Dec 21;318(5858):1917-20
pubmed: 18029452
Stem Cell Res Ther. 2015 Mar 26;6:48
pubmed: 25889591
BMJ. 2007 Jan 27;334(7586):177
pubmed: 17255594