A highly efficient heptamethine cyanine antenna for photosynthetic Reaction Center: From chemical design to ultrafast energy transfer investigation of the hybrid system.

Bacterial Proteins / chemistry Energy Transfer Fluorescence Light Light-Harvesting Protein Complexes / chemistry Rhodobacter sphaeroides / enzymology
Bioconjugation Biophotovoltaic Light harvesting antenna Photocurrent Photoenzyme Photosynthetic bacteria Solar energy conversion

Journal

Biochimica et biophysica acta. Bioenergetics
ISSN: 1879-2650
Titre abrégé: Biochim Biophys Acta Bioenerg
Pays: Netherlands
ID NLM: 101731706

Informations de publication

Date de publication:
01 04 2019
Historique:
received: 03 07 2018
revised: 11 12 2018
accepted: 26 01 2019
pubmed: 6 2 2019
medline: 28 8 2019
entrez: 6 2 2019
Statut: ppublish

Résumé

The photosynthetic Reaction Center (RC) from the purple bacterium Rhodobacter sphaeroides has unique photoconversion capabilities, that can be exploited in assembly biohybrid devices for applications in solar energy conversion. Extending the absorption cross section of isolated RC through covalent functionalization with ad-hoc synthesized artificial antennas is a successful strategy to outperform the efficiency of the pristine photoenzyme under visible light excitation. Here we report a new heptamethine cyanine antenna that, upon covalent binding to RC, forms a biohybrid (hCyN7-RC) which, under white light excitation, has doubled photoconversion efficiency versus the bare photoenzyme. The artificial antenna hCyN7 successfully meets appropriate optical properties, i.e. peak position of absorption and emission maximum in the visible and NIR region respectively, large Stokes shift, and high fluorescence quantum yield, required for improving the efficiency of the biohybrid in the production of the charge-separated state in the RC. The kinetics of energy transfer and charge separation of hCyN7-RC studied via ultrafast visible and IR spectroscopies are here presented. The antenna transfers energy to RC chromophores within <10 ps and the rate of Q

Identifiants

DOI: 10.1016/j.bbabio.2019.01.009 PMID: 30721661
pubmed: 30721661
pii: S0005-2728(18)30185-3
doi: 10.1016/j.bbabio.2019.01.009
pii:
doi:

Substances chimiques

Bacterial Proteins 0
Light-Harvesting Protein Complexes 0

Types de publication

Journal Article Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Pagination

350-359

Informations de copyright

Copyright © 2019 Elsevier B.V. All rights reserved.

Auteurs

Simona la Gatta (S)

Dipartimento di Chimica, Università degli Studi di Bari "Aldo Moro", Via Orabona 4, 70125 Bari, Italy; CNR-IPCF, Institute for Physical and Chemical Processes, Bari unit, Via Orabona 4, 70125 Bari, Italy.

Francesco Milano (F)

CNR-IPCF, Institute for Physical and Chemical Processes, Bari unit, Via Orabona 4, 70125 Bari, Italy.

Gianluca M Farinola (GM)

Dipartimento di Chimica, Università degli Studi di Bari "Aldo Moro", Via Orabona 4, 70125 Bari, Italy.

Angela Agostiano (A)

Dipartimento di Chimica, Università degli Studi di Bari "Aldo Moro", Via Orabona 4, 70125 Bari, Italy; CNR-IPCF, Institute for Physical and Chemical Processes, Bari unit, Via Orabona 4, 70125 Bari, Italy.

Mariangela Di Donato (M)

LENS (European Laboratory for Nonlinear Spectroscopy), via N. Carrara 1, 50019 Sesto Fiorentino, FI, Italy; INO (Istituto Nazionale di Ottica), Largo Fermi 6, 50125 Firenze, Italy.

Andrea Lapini (A)

LENS (European Laboratory for Nonlinear Spectroscopy), via N. Carrara 1, 50019 Sesto Fiorentino, FI, Italy; INO (Istituto Nazionale di Ottica), Largo Fermi 6, 50125 Firenze, Italy.

Paolo Foggi (P)

LENS (European Laboratory for Nonlinear Spectroscopy), via N. Carrara 1, 50019 Sesto Fiorentino, FI, Italy; INO (Istituto Nazionale di Ottica), Largo Fermi 6, 50125 Firenze, Italy; Department of Chemistry, University of Perugia, via Elce di Sotto 8, 06100 Perugia, Italy.

Massimo Trotta (M)

CNR-IPCF, Institute for Physical and Chemical Processes, Bari unit, Via Orabona 4, 70125 Bari, Italy. Electronic address: massimo.trotta@cnr.it.

Roberta Ragni (R)

Dipartimento di Chimica, Università degli Studi di Bari "Aldo Moro", Via Orabona 4, 70125 Bari, Italy. Electronic address: roberta.ragni@uniba.it.

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Classifications MeSH

questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Protéines bactériennes › A highly efficient heptamethine cyanine...
questionsmedicales.fr › Phénomènes chimiques › Transfert d'énergie › A highly efficient heptamethine cyanine...
questionsmedicales.fr › Phénomènes physiques › Phénomènes optiques › Fluorescence › A highly efficient heptamethine cyanine...
questionsmedicales.fr › Phénomènes physiques › Rayonnement › Rayonnement non ionisant › Lumière › A highly efficient heptamethine cyanine...
questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Protéines végétales › Protéines chloroplastiques › Protéines de la membrane thylakoïdale › Complexe protéique du centre réactionnel de la photosynthèse › Complexes collecteurs de lumière › A highly efficient heptamethine cyanine...
questionsmedicales.fr › Bactéries › Proteobacteria › Alphaproteobacteria › Rhodobacteraceae › Rhodobacter › Rhodobacter sphaeroides › A highly efficient heptamethine cyanine...