Heterologous expression and characterization of a new galactose-binding lectin from Bauhinia forficata with antiproliferative activity.
Antiproliferative activity
Bauhinia lectins
HT-29
MCF-7
Journal
International journal of biological macromolecules
ISSN: 1879-0003
Titre abrégé: Int J Biol Macromol
Pays: Netherlands
ID NLM: 7909578
Informations de publication
Date de publication:
01 May 2019
01 May 2019
Historique:
received:
29
10
2018
revised:
07
01
2019
accepted:
18
01
2019
pubmed:
6
2
2019
medline:
20
6
2019
entrez:
6
2
2019
Statut:
ppublish
Résumé
In this study, we describe the characterization of a new lectin, BfL-II, purified from the seeds of Bauhinia forficata, which is distinct, at sequence-level, from the previously reported lectin from the same specie (BfL). In addition, the gene for this lectin was cloned and expressed in Escherichia coli and its antiproliferative activity was evaluated against human breast and colorectal cancer cells (MCF-7 and HT-29, respectively). The treatment with 100 μg/μL of either native or recombinant BfL (nBfL or rBfL) significantly reduced the proliferation of both cancer cell lines (p < 0.01). The inhibition of HT-29 cell proliferation was as high as 82.5% and 93.6% with 100 μg/μL of rBfL-I and nBfL after 24 h, respectively. Therefore, BfL-II presents a promising antiproliferative activity, which may be applied in the development of new anticancer treatments.
Identifiants
pubmed: 30721748
pii: S0141-8130(18)35734-9
doi: 10.1016/j.ijbiomac.2019.01.090
pii:
doi:
Substances chimiques
Antineoplastic Agents
0
Galectins
0
Plant Lectins
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
877-884Informations de copyright
Copyright © 2019. Published by Elsevier B.V.