Comparative study of chikungunya Virus-Like Particles and Pseudotyped-Particles used for serological detection of specific immunoglobulin M.
Arbovirus
Chikungunya
Field-flow fractionation
Pseudotyped virus
Virus-like particles
Journal
Virology
ISSN: 1096-0341
Titre abrégé: Virology
Pays: United States
ID NLM: 0110674
Informations de publication
Date de publication:
03 2019
03 2019
Historique:
received:
20
09
2018
revised:
28
01
2019
accepted:
29
01
2019
pubmed:
6
2
2019
medline:
16
7
2019
entrez:
6
2
2019
Statut:
ppublish
Résumé
The incidence of chikungunya virus (CHIKV) infection has increased dramatically in recent decades. Effective diagnostic methods must be available to optimize patient management. IgM-capture Enzyme-Linked Immunosorbent Assay (MAC-ELISA) is routinely used for the detection of specific CHIKV IgM. This method requires inactivated CHIKV viral lysate (VL). The use of viral bioparticles such as Virus-Like Particles (VLPs) and Pseudotyped-Particles (PPs) could represent an alternative to VL. Bioparticles performances were established by MAC-ELISA; physico-chemical characterizations were performed by field-flow fractionation (HF5) and confirmed by electron microscopy. Non-purified PPs give a detection signal higher than for VL. Results suggested that the signal difference observed in MAC-ELISA was probably due to the intrinsic antigenic properties of particles. The use of CHIKV bioparticles such as VLPs and PPs represents an attractive alternative to VL. Compared to VL and VLPs, non-purified PPs have proven to be more powerful antigens for specific IgM capture.
Identifiants
pubmed: 30721816
pii: S0042-6822(19)30030-3
doi: 10.1016/j.virol.2019.01.027
pii:
doi:
Substances chimiques
Antibodies, Viral
0
Immunoglobulin M
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
195-204Informations de copyright
Copyright © 2019 Elsevier Inc. All rights reserved.