Amelioration of Ductular Reaction by Stem Cell Derived Extracellular Vesicles in MDR2 Knockout Mice via Lethal-7 microRNA.
ATP Binding Cassette Transporter, Subfamily B
/ metabolism
Animals
Cell Differentiation
/ physiology
Cells, Cultured
/ cytology
Cholangitis, Sclerosing
/ metabolism
Disease Models, Animal
Female
Hepatocytes
/ cytology
Humans
Liver Cirrhosis
/ metabolism
Mice
Mice, Knockout
MicroRNAs
/ metabolism
Random Allocation
Real-Time Polymerase Chain Reaction
/ methods
Risk Factors
Sensitivity and Specificity
Stem Cells
/ cytology
ATP-Binding Cassette Sub-Family B Member 4
Journal
Hepatology (Baltimore, Md.)
ISSN: 1527-3350
Titre abrégé: Hepatology
Pays: United States
ID NLM: 8302946
Informations de publication
Date de publication:
06 2019
06 2019
Historique:
received:
23
07
2018
accepted:
15
01
2019
pubmed:
7
2
2019
medline:
17
6
2020
entrez:
7
2
2019
Statut:
ppublish
Résumé
Cholangiopathies are diseases that affect cholangiocytes, the cells lining the biliary tract. Liver stem cells (LSCs) are able to differentiate into all cells of the liver and possibly influence the surrounding liver tissue by secretion of signaling molecules. One way in which cells can interact is through secretion of extracellular vesicles (EVs), which are small membrane-bound vesicles that contain proteins, microRNAs (miRNAs), and cytokines. We evaluated the contents of liver stem cell-derived EVs (LSCEVs), compared their miRNA contents to those of EVs isolated from hepatocytes, and evaluated the downstream targets of these miRNAs. We finally evaluated the crosstalk among LSCs, cholangiocytes, and human hepatic stellate cells (HSCs). We showed that LSCEVs were able to reduce ductular reaction and biliary fibrosis in multidrug resistance protein 2 (MDR2)
Identifiants
pubmed: 30723922
doi: 10.1002/hep.30542
pmc: PMC7015419
mid: NIHMS1067229
doi:
Substances chimiques
ATP Binding Cassette Transporter, Subfamily B
0
MicroRNAs
0
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, Non-P.H.S.
Langues
eng
Sous-ensembles de citation
IM
Pagination
2562-2578Subventions
Organisme : NIDDK NIH HHS
ID : R01 DK108959
Pays : United States
Organisme : NIDDK NIH HHS
ID : R01 DK095862
Pays : United States
Organisme : NIAAA NIH HHS
ID : R21 AA025997
Pays : United States
Organisme : VA
Pays : United States
Organisme : NIDDK NIH HHS
ID : R01 DK058411
Pays : United States
Organisme : U.S. Department of Veterans Affairs
ID : 1I01BX0
Pays : International
Organisme : BLRD VA
ID : I01 BX003031
Pays : United States
Organisme : NIDDK NIH HHS
ID : R01 DK115184
Pays : United States
Organisme : NIAAA NIH HHS
ID : R21 AA025157
Pays : United States
Organisme : NIDDK NIH HHS
ID : R01 DK110035
Pays : United States
Organisme : NIDDK NIH HHS
ID : R01 DK054811
Pays : United States
Organisme : U.S. Department of Veterans Affairs
ID : 5I01BX000574
Pays : International
Organisme : BLRD VA
ID : I01 BX000574
Pays : United States
Organisme : BLRD VA
ID : I01 BX001724
Pays : United States
Organisme : U.S. Department of Veterans Affairs
ID : 5I01BX002192
Pays : International
Organisme : NIDDK NIH HHS
ID : R01 DK076898
Pays : United States
Organisme : U.S. Department of Veterans Affairs
ID : 5I01 BX001724
Pays : International
Organisme : BLRD VA
ID : IK6 BX004601
Pays : United States
Informations de copyright
© 2019 by the American Association for the Study of Liver Diseases.
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