Macrophages significantly enhance wound healing in a vascularized skin model.
hydrogel
macrophages
skin graft
vascularized tissue
wound healing
Journal
Journal of biomedical materials research. Part A
ISSN: 1552-4965
Titre abrégé: J Biomed Mater Res A
Pays: United States
ID NLM: 101234237
Informations de publication
Date de publication:
06 2019
06 2019
Historique:
received:
30
11
2018
revised:
17
01
2019
accepted:
29
01
2019
pubmed:
7
2
2019
medline:
15
7
2020
entrez:
7
2
2019
Statut:
ppublish
Résumé
Tissue-engineered dermo-epidermal skin grafts could be applied for the treatment of large skin wounds or used as an in vitro wound-healing model. However, there is currently no skin replacement model that includes both, endothelial cells to simulate vascularization, and macrophages to regulate wound healing and tissue regeneration. Here, we describe for the first time a tissue-engineered, fully vascularized dermo-epidermal skin graft based on a fibrin hydrogel scaffold, using exclusively human primary cells. We show that endothelial cells and human dermal fibroblasts form capillary-like structures within the dermis whereas keratinocytes form the epithelial cell layer. Macrophages played a key role in controlling the number of epithelial cells and their morphology after skin injury induced with a CO
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
1340-1350Subventions
Organisme : Mye Euniter
ID : BM1404
Pays : International
Organisme : START Program of the Medical Faculty RWTH Aachen University funding by University Hospital Aachen, Germany
ID : 694300, initials: 11/14
Pays : International
Organisme : RWTH Aachen University
Pays : International
Organisme : Medical Faculty of the RWTH Aachen University
Pays : International
Organisme : Horizon 2020
Pays : International
Informations de copyright
© 2019 Wiley Periodicals, Inc.