Recombinant Methioninase as a DNA Demethylation Agent.

Azacitidine / pharmacology Carbon-Sulfur Lyases / pharmacology Cell Line, Tumor DNA Demethylation / drug effects DNA Methylation / drug effects Humans Recombinant Proteins / pharmacology
Cancer cells DNA hypomethylation Malignancy Recombinant methioninase

Journal

Methods in molecular biology (Clifton, N.J.)
ISSN: 1940-6029
Titre abrégé: Methods Mol Biol
Pays: United States
ID NLM: 9214969

Informations de publication

Date de publication:
2019
Historique:
entrez: 7 2 2019
pubmed: 7 2 2019
medline: 28 5 2019
Statut: ppublish

Résumé

This chapter reviews the effect of methionine (MET) restriction, via treatment with recombinant methioninase (rMETase), on DNA methylation of cancer cells. CCRF-CEM human cancer cells were treated with rMETase under subcytotoxic conditions. The rMETase-treated cells contained significantly lower levels of genomic methylated DNA than did untreated control cells. DNA methylation was measured by incorporation of the methyl group of [

Identifiants

DOI: 10.1007/978-1-4939-8796-2_22 PMID: 30725424
pubmed: 30725424
doi: 10.1007/978-1-4939-8796-2_22
doi:

Substances chimiques

Recombinant Proteins 0
Carbon-Sulfur Lyases EC 4.4.-
L-methionine gamma-lyase EC 4.4.1.11
Azacitidine M801H13NRU

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Pagination

279-284

Auteurs

Robert M Hoffman (RM)

AntiCancer, Inc., San Diego, CA, USA. all@anticancer.com.
Department of Surgery, University of California, San Diego, CA, USA. all@anticancer.com.

David Machover (D)

Hopital Paul Brousse, Villejuif Cedex, France.

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Classifications MeSH

questionsmedicales.fr › Acides nucléiques, nucléotides et nucléosides › Nucléosides › Ribonucléosides › Cytidine › Azacitidine › Recombinant Methioninase as a DNA Demethylation Agent.
questionsmedicales.fr › Enzymes et coenzymes › Enzymes › Lyases › Carbon-sulfur lyases › Recombinant Methioninase as a DNA Demethylation Agent.
questionsmedicales.fr › Cellules › Cellules cultivées › Cellules cancéreuses en culture › Lignée cellulaire tumorale › Recombinant Methioninase as a DNA Demethylation Agent.
questionsmedicales.fr › Métabolisme › Désalkylation › Déméthylation › Déméthylation de l'ADN › Recombinant Methioninase as a DNA Demethylation Agent.
questionsmedicales.fr › Phénomènes génétiques › Méthylation de l'ADN › Recombinant Methioninase as a DNA Demethylation Agent.
questionsmedicales.fr › Eucaryotes › Animaux › Chordés › Vertébrés › Mammifères › Eutheria › Primates › Haplorhini › Catarrhini › Hominidae › Humains › Recombinant Methioninase as a DNA Demethylation Agent.
questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Protéines recombinantes › Recombinant Methioninase as a DNA Demethylation Agent.