C3P3-G1: first generation of a eukaryotic artificial cytoplasmic expression system.

Animals CHO Cells Cell Nucleus / genetics Cricetulus Cytoplasm / chemistry DNA-Directed RNA Polymerases / chemistry Eukaryotic Cells / chemistry Humans Poly A / genetics Polyadenylation / genetics Transcription, Genetic

Journal

Nucleic acids research
ISSN: 1362-4962
Titre abrégé: Nucleic Acids Res
Pays: England
ID NLM: 0411011

Informations de publication

Date de publication:
18 03 2019
Historique:
accepted: 25 01 2019
revised: 03 12 2018
received: 11 11 2018
pubmed: 7 2 2019
medline: 16 10 2019
entrez: 7 2 2019
Statut: ppublish

Résumé

Most eukaryotic expression systems make use of host-cell nuclear transcriptional and post-transcriptional machineries. Here, we present the first generation of the chimeric cytoplasmic capping-prone phage polymerase (C3P3-G1) expression system developed by biological engineering, which generates capped and polyadenylated transcripts in host-cell cytoplasm by means of two components. First, an artificial single-unit chimeric enzyme made by fusing an mRNA capping enzyme and a DNA-dependent RNA polymerase. Second, specific DNA templates designed to operate with the C3P3-G1 enzyme, which encode for the transcripts and their artificial polyadenylation. This system, which can potentially be adapted to any in cellulo or in vivo eukaryotic expression applications, was optimized for transient expression in mammalian cells. C3P3-G1 shows promising results for protein production in Chinese Hamster Ovary (CHO-K1) cells. This work also provides avenues for enhancing the performances for next generation C3P3 systems.

Identifiants

DOI: 10.1093/nar/gkz069 PMID: 30726994 PMC: PMC6412113
pubmed: 30726994
pii: 5307548
doi: 10.1093/nar/gkz069
pmc: PMC6412113
doi:

Substances chimiques

Poly A 24937-83-5
DNA-Directed RNA Polymerases EC 2.7.7.6

Types de publication

Journal Article Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Pagination

2681-2698

Informations de copyright

© The Author(s) 2019. Published by Oxford University Press on behalf of Nucleic Acids Research.

Références

Nat Struct Mol Biol. 2007 May;14(5):449-51
pubmed: 17417654
EMBO J. 2007 Nov 28;26(23):4913-25
pubmed: 17989694
Virology. 1993 Mar;193(1):319-28
pubmed: 8382399
Structure. 2008 Apr;16(4):501-12
pubmed: 18400173
J Biol Chem. 1978 Jun 25;253(12):4490-8
pubmed: 659429
Mol Cell. 1999 Mar;3(3):405-11
pubmed: 10198643
Nucleic Acids Res. 2012 Jun;40(11):5023-33
pubmed: 22344696
Trends Biochem Sci. 2003 Apr;28(4):215-20
pubmed: 12713906
J Virol. 1978 Nov;28(2):444-9
pubmed: 214572
Proc Natl Acad Sci U S A. 1986 Nov;83(21):8122-6
pubmed: 3095828
J Biol Chem. 1995 Aug 11;270(32):18997-9007
pubmed: 7642560
J Biol Chem. 1995 Feb 10;270(6):2800-8
pubmed: 7852352
Blood. 1996 Mar 1;87(5):1746-53
pubmed: 8634420
J Biol Chem. 2005 Mar 4;280(9):7823-8
pubmed: 15632160
Nat Struct Mol Biol. 2004 Feb;11(2):121-7
pubmed: 14749774
J Biol Chem. 1984 May 25;259(10):6575-8
pubmed: 6327686
Gene Ther. 2006 Jul;13(13):1048-51
pubmed: 16525478
Nucleic Acids Res. 2004 Jan 15;32(1):e14
pubmed: 14744982
Virology. 1998 Apr 10;243(2):331-9
pubmed: 9568032
Microbiol Mol Biol Rev. 1999 Jun;63(2):405-45
pubmed: 10357856
Genes Dev. 1991 Nov;5(11):2108-16
pubmed: 1682219
Nature. 2004 Jul 8;430(6996):223-6
pubmed: 15241417
Biotechniques. 1996 Jan;20(1):136-41
pubmed: 8770418
Protein Sci. 2001 Mar;10(3):649-55
pubmed: 11344333
Trends Mol Med. 2003 Feb;9(2):67-72
pubmed: 12615040
Proc Natl Acad Sci U S A. 1982 Jan;79(2):422-6
pubmed: 6952193
Syst Synth Biol. 2010 Sep;4(3):215-25
pubmed: 21189842
Nature. 2010 Nov 18;468(7322):452-6
pubmed: 21085181
Proc Natl Acad Sci U S A. 1995 Dec 19;92(26):12250-4
pubmed: 8618879
J Mol Biol. 1989 Mar 20;206(2):333-48
pubmed: 2497259
CRC Crit Rev Biochem. 1981;10(1):1-38
pubmed: 6111419
Mol Cell Biol. 1990 Jan;10(1):353-60
pubmed: 2403641
Nucleic Acids Res. 2007;35(4):e26
pubmed: 17259217
Proc Natl Acad Sci U S A. 1990 Jun;87(11):4023-7
pubmed: 2161527
J Bacteriol. 2002 May;184(10):2833-6
pubmed: 11976314
J Biol Chem. 2005 Apr 1;280(13):13153-62
pubmed: 15677480
Curr Chem Genomics. 2009 Jun 11;3:33-41
pubmed: 20161834
J Biol Chem. 1992 Jun 5;267(16):11322-8
pubmed: 1597465
J Biol Chem. 1988 Nov 25;263(33):17516-21
pubmed: 3182860
Toxicol In Vitro. 2008 Jun;22(4):1099-106
pubmed: 18400464
Plasmid. 1998 Nov;40(3):243-6
pubmed: 9806862
Genes Dev. 1997 Dec 15;11(24):3306-18
pubmed: 9407024
RNA. 2000 Apr;6(4):584-97
pubmed: 10786849
Mol Cell Biol. 1986 Nov;6(11):4077-87
pubmed: 3025630
Annu Rev Biochem. 1993;62:893-930
pubmed: 8352604
Nat Rev Mol Cell Biol. 2001 Apr;2(4):237-46
pubmed: 11283721
Nat Rev Genet. 2014 Aug;15(8):541-55
pubmed: 25022906
Yeast. 2001 Jun 30;18(9):815-25
pubmed: 11427964
J Virol. 2017 May 12;91(11):
pubmed: 28298603
Nature. 2000 Sep 28;407(6803):471-5
pubmed: 11028991
EMBO J. 1998 Apr 1;17(7):2107-21
pubmed: 9524132
Nature. 2017 Jan 19;541(7637):371-375
pubmed: 28002401
J Virol. 2001 Jan;75(2):782-8
pubmed: 11134291
J Virol. 1977 Feb;21(2):475-83
pubmed: 833934
Cancer Res. 1991 Jan 1;51(1):341-8
pubmed: 1824823
Nat Struct Mol Biol. 2007 Sep;14(9):796-806
pubmed: 17676063
RNA. 2001 Sep;7(9):1192-212
pubmed: 11565744
J Biol Chem. 1980 Dec 10;255(23):11588-98
pubmed: 6254974
Nat Immunol. 2004 Jul;5(7):730-7
pubmed: 15208624
Biochemistry. 1997 Mar 11;36(10):2908-18
pubmed: 9062120
J Biol Chem. 1994 Sep 30;269(39):24472-9
pubmed: 7929111
Mol Cell Biol. 1995 Nov;15(11):6222-31
pubmed: 7565775
Nat Rev Microbiol. 2011 Dec 05;10(1):51-65
pubmed: 22138959
Eur J Biochem. 1997 Aug 1;247(3):741-53
pubmed: 9288893
Cell. 2012 Jun 22;149(7):1635-46
pubmed: 22608085
Science. 2002 Nov 15;298(5597):1387-95
pubmed: 12242451
Blood. 2001 Dec 15;98(13):3626-34
pubmed: 11739166
Biochemistry. 1991 Jun 25;30(25):6305-13
pubmed: 2059636
J Biol Chem. 2009 Mar 27;284(13):8692-702
pubmed: 19176527
J Biochem Biophys Methods. 2001 Oct 30;49(1-3):455-65
pubmed: 11694294
Bioinformatics. 2004 Mar 22;20(5):629-35
pubmed: 15033869
Proc Natl Acad Sci U S A. 1990 Sep;87(17):6743-7
pubmed: 2204064
RNA. 2002 May;8(5):686-97
pubmed: 12022234
Gene Ther. 1996 Nov;3(11):1010-7
pubmed: 9044741
Methods Mol Biol. 2008;419:215-30
pubmed: 18369986
Genes Dev. 1989 Sep;3(9):1401-12
pubmed: 2575065
Proc Natl Acad Sci U S A. 1998 Nov 10;95(23):13537-42
pubmed: 9811835
Science. 2006 Nov 10;314(5801):994-7
pubmed: 17038590
Biochemistry. 2003 Dec 23;42(50):14957-67
pubmed: 14674772
Adv Drug Deliv Rev. 2001 Nov 19;52(3):219-26
pubmed: 11718946
J Cell Biol. 1990 Feb;110(2):283-94
pubmed: 2298808
Proc Natl Acad Sci U S A. 2007 Nov 6;104(45):17620-5
pubmed: 17978174
Proc Natl Acad Sci U S A. 2012 Jun 12;109(24):9372-7
pubmed: 22635272
J Mol Biol. 1998 Jul 31;280(5):859-66
pubmed: 9671555
BMC Bioinformatics. 2009 Dec 15;10:421
pubmed: 20003500

Auteurs

Philippe H Jaïs (PH)

Eukarÿs SAS, Génopole Campus 3, 4 rue Pierre Fontaine, 91058 Evry Cedex, France.

Etienne Decroly (E)

Architecture et Fonction des Macromolécules Biologiques (AFMB) UMR 7257 CNRS/AMU, 163 Avenue de Luminy, 13288 Marseille Cedex 9, France.

Eric Jacquet (E)

Institut de Chimie des Substances Naturelles, CNRS UPR2301, Université Paris-Saclay, Avenue de la Terrasse, 91198 Gif-sur-Yvette, France.

Marine Le Boulch (M)

Eukarÿs SAS, Génopole Campus 3, 4 rue Pierre Fontaine, 91058 Evry Cedex, France.

Aurélien Jaïs (A)

Eukarÿs SAS, Génopole Campus 3, 4 rue Pierre Fontaine, 91058 Evry Cedex, France.

Olivier Jean-Jean (O)

Sorbonne Université, CNRS-UMR8256, Biological Adaptation and Ageing, Institut de Biologie Paris Seine (B2A-IBPS), F-75252 Paris, France.

Heather Eaton (H)

Medical Microbiology and Immunology, University of Alberta, 6-142J Katz Group Centre for Pharmacy and Health Research, 114 Street NW, Edmonton, Alberta T6G 2E1, Canada.

Prishila Ponien (P)

Institut de Chimie des Substances Naturelles, CNRS UPR2301, Université Paris-Saclay, Avenue de la Terrasse, 91198 Gif-sur-Yvette, France.

Fréderique Verdier (F)

INSERM Unit 1016, Institut Cochin, Bâtiment Gustave Roussy, 27 rue du faubourg Saint-Jacques, 75014 Paris, France.

Bruno Canard (B)

Architecture et Fonction des Macromolécules Biologiques (AFMB) UMR 7257 CNRS/AMU, 163 Avenue de Luminy, 13288 Marseille Cedex 9, France.

Sergio Goncalves (S)

Eukarÿs SAS, Génopole Campus 3, 4 rue Pierre Fontaine, 91058 Evry Cedex, France.

Stéphane Chiron (S)

Eukarÿs SAS, Génopole Campus 3, 4 rue Pierre Fontaine, 91058 Evry Cedex, France.

Maude Le Gall (M)

Gastrointestinal and Metabolic Dysfunctions in Nutritional Pathologies, INSERM UMRS1149, 16 rue Henri Huchard, 75890 Paris Cedex 18, France.

Patrick Mayeux (P)

INSERM Unit 1016, Institut Cochin, Bâtiment Gustave Roussy, 27 rue du faubourg Saint-Jacques, 75014 Paris, France.

Maya Shmulevitz (M)

Medical Microbiology and Immunology, University of Alberta, 6-142J Katz Group Centre for Pharmacy and Health Research, 114 Street NW, Edmonton, Alberta T6G 2E1, Canada.

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Classifications MeSH

questionsmedicales.fr › Eucaryotes › Animaux › C3P3-G1: first generation of a eukaryotic...
questionsmedicales.fr › Cellules › Cellules épithéliales › Cellules CHO › C3P3-G1: first generation of a eukaryotic...
questionsmedicales.fr › Cellules › Structures cellulaires › Espace intracellulaire › Cytoplasme › Structures cytoplasmiques › Organites › Noyau de la cellule › C3P3-G1: first generation of a eukaryotic...
questionsmedicales.fr › Eucaryotes › Animaux › Chordés › Vertébrés › Mammifères › Eutheria › Rodentia › Muridae › Arvicolinae › Cricetinae › Cricetulus › C3P3-G1: first generation of a eukaryotic...
questionsmedicales.fr › Cellules › Structures cellulaires › Espace intracellulaire › Cytoplasme › C3P3-G1: first generation of a eukaryotic...
questionsmedicales.fr › Enzymes et coenzymes › Enzymes › Transferases › Phosphotransferases › Nucleotidyltransferases › RNA nucleotidyltransferases › DNA-directed RNA polymerases › C3P3-G1: first generation of a eukaryotic...
questionsmedicales.fr › Cellules › Cellules eucaryotes › C3P3-G1: first generation of a eukaryotic...
questionsmedicales.fr › Eucaryotes › Animaux › Chordés › Vertébrés › Mammifères › Eutheria › Primates › Haplorhini › Catarrhini › Hominidae › Humains › C3P3-G1: first generation of a eukaryotic...
questionsmedicales.fr › Acides nucléiques, nucléotides et nucléosides › Nucléotides › Polynucléotides › Polyribonucléotides › Poly A › C3P3-G1: first generation of a eukaryotic...
questionsmedicales.fr › Phénomènes génétiques › Régulation de l'expression des gènes › Maturation post-transcriptionnelle des ARN › Maturation de l'extrémité 3' des ARN › Polyadénylation › C3P3-G1: first generation of a eukaryotic...
questionsmedicales.fr › Phénomènes génétiques › Expression des gènes › Transcription génétique › C3P3-G1: first generation of a eukaryotic...