Analysis of mRNA, miRNA, and DNA in Bone Cells by RT-qPCR and In Situ Hybridization.
Animals
Bone and Bones
/ cytology
Cell Line, Tumor
DNA
/ genetics
Digoxigenin
/ chemistry
Gene Expression Profiling
/ instrumentation
Histocytological Preparation Techniques
/ instrumentation
Humans
In Situ Hybridization
/ instrumentation
Mice
Mice, Nude
MicroRNAs
/ genetics
Oligonucleotides
/ chemistry
RNA, Messenger
/ genetics
Real-Time Polymerase Chain Reaction
/ instrumentation
Reverse Transcriptase Polymerase Chain Reaction
/ instrumentation
Xenograft Model Antitumor Assays
Bone
Bone sarcoma
Chromogenic in situ hybridization
DNA
Gene expression
Histology
LNA probes
Primers
RT-qPCR
mRNA
miRNA
Journal
Methods in molecular biology (Clifton, N.J.)
ISSN: 1940-6029
Titre abrégé: Methods Mol Biol
Pays: United States
ID NLM: 9214969
Informations de publication
Date de publication:
2019
2019
Historique:
entrez:
8
2
2019
pubmed:
8
2
2019
medline:
14
6
2019
Statut:
ppublish
Résumé
The aim of this chapter is to describe a method used to evaluate gene expression and microRNAs (miRNAs) in bone cells or tissue using Reverse transcription and quantitative Polymerase Chain Reaction (RT-qPCR), and a method to assess chromogenic in situ hybridization (CISH) on Formalin Fixed Paraffin Embedded (FFPE ) mouse bone tissue to detect both DNA and mRNA transcripts using the double digoxigenin (DIG) locked nucleic acid (LNA™) probes .
Identifiants
pubmed: 30729465
doi: 10.1007/978-1-4939-8997-3_9
doi:
Substances chimiques
MicroRNAs
0
Oligonucleotides
0
RNA, Messenger
0
locked nucleic acid
0
DNA
9007-49-2
Digoxigenin
NQ1SX9LNAU
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM