The protective effect of epigallocatechin 3-gallate on mouse pancreatic islets via the Nrf2 pathway.
Animals
Blood Glucose
Catechin
/ analogs & derivatives
Cell Survival
Gene Expression
/ drug effects
Heme Oxygenase-1
/ genetics
In Vitro Techniques
Insulin Secretion
/ drug effects
Islets of Langerhans
/ cytology
Islets of Langerhans Transplantation
Male
Mice, Inbred C57BL
Mice, Inbred ICR
NF-E2-Related Factor 2
/ genetics
RNA, Messenger
/ genetics
Reactive Oxygen Species
/ metabolism
Tissue Culture Techniques
Transplantation Conditioning
Epigallocatechin 3-gallate (EGCG)
Islet transplantation
Nuclear factor erythroid 2-related factor 2 (Nrf2)
Pre-conditioning
Reactive oxygen species (ROS) production
Journal
Surgery today
ISSN: 1436-2813
Titre abrégé: Surg Today
Pays: Japan
ID NLM: 9204360
Informations de publication
Date de publication:
Jun 2019
Jun 2019
Historique:
received:
26
09
2018
accepted:
20
12
2018
pubmed:
8
2
2019
medline:
14
6
2019
entrez:
8
2
2019
Statut:
ppublish
Résumé
Epigallocatechin 3-gallate (EGCG), a green tea polyphenol, has been shown to have anti-oxidant and anti-inflammatory effects in vitro and in vivo. The aim of this study was to investigate the effects and mechanism of EGCG on isolated pancreatic islets as pre-conditioning for pancreatic islet transplantation. The pancreatic islets were divided into two groups: an islet culture medium group (control) and an islet culture medium with EGCG (100 µM) group. We investigated the islet viability, Nrf2 expression, reactive oxygen species (ROS) production, and heme oxygenase-1 (HO-1) mRNA. Five hundred islet equivalents after 12 h of culture for the EGCG 100 µM and control group were transplanted under the kidney capsule of streptozotocin-induced diabetic ICR mice. The cell viability and insulin secretion ability in the EGCG group were preserved, and the nuclear translocation of Nrf2 was increased in the EGCG group (p < 0.01). While the HO-1 mRNA levels were also higher in the EGCG group than in the control group (p < 0.05), the ROS production was lower (p < 0.01). An in vivo functional assessment showed that the blood glucose level had decreased in the EGCG group after transplantation (p < 0.01). EGCG protects the viability and function of islets by suppressing ROS production via the Nrf2 pathway.
Identifiants
pubmed: 30730004
doi: 10.1007/s00595-019-1761-0
pii: 10.1007/s00595-019-1761-0
doi:
Substances chimiques
Blood Glucose
0
NF-E2-Related Factor 2
0
Nfe2l2 protein, mouse
0
RNA, Messenger
0
Reactive Oxygen Species
0
Catechin
8R1V1STN48
epigallocatechin gallate
BQM438CTEL
Heme Oxygenase-1
EC 1.14.14.18
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
536-545Références
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