Structurally defined signaling in neuro-glia units in the enteric nervous system.
Ca2+ imaging
Ca2+ uncaging
enteric glial cell
enteric neuron
gastrointestinal tract
purinergic signaling
synaptic
Journal
Glia
ISSN: 1098-1136
Titre abrégé: Glia
Pays: United States
ID NLM: 8806785
Informations de publication
Date de publication:
06 2019
06 2019
Historique:
received:
10
08
2018
revised:
11
01
2019
accepted:
11
01
2019
pubmed:
8
2
2019
medline:
31
12
2019
entrez:
8
2
2019
Statut:
ppublish
Résumé
Coordination of gastrointestinal function relies on joint efforts of enteric neurons and glia, whose crosstalk is vital for the integration of their activity. To investigate the signaling mechanisms and to delineate the spatial aspects of enteric neuron-to-glia communication within enteric ganglia we developed a method to stimulate single enteric neurons while monitoring the activity of neighboring enteric glial cells. We combined cytosolic calcium uncaging of individual enteric neurons with calcium imaging of enteric glial cells expressing a genetically encoded calcium indicator and demonstrate that enteric neurons signal to enteric glial cells through pannexins using paracrine purinergic pathways. Sparse labeling of enteric neurons and high-resolution analysis of the structural relation between neuronal cell bodies, varicose release sites and enteric glia uncovered that this form of neuron-to-glia communication is contained between the cell body of an enteric neuron and its surrounding enteric glial cells. Our results reveal the spatial and functional foundation of neuro-glia units as an operational cellular assembly in the enteric nervous system.
Identifiants
pubmed: 30730592
doi: 10.1002/glia.23596
pmc: PMC6593736
doi:
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
1167-1178Informations de copyright
© 2019 The Authors. Glia published by Wiley Periodicals, Inc.
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