Synthesis and Fabrication of Surface-Active Microparticles Using a Membrane Emulsion Technique and Conjugation of Model Protein via Strain-Promoted Azide-Alkyne Click Chemistry in Physiological Conditions.
Journal
Bioconjugate chemistry
ISSN: 1520-4812
Titre abrégé: Bioconjug Chem
Pays: United States
ID NLM: 9010319
Informations de publication
Date de publication:
20 03 2019
20 03 2019
Historique:
pubmed:
8
2
2019
medline:
30
11
2019
entrez:
8
2
2019
Statut:
ppublish
Résumé
The rapid surface immobilization of protein on monodispersed polyester microcarriers is reported. A model protein, functionalized with a dibenzocyclooctyne core, immobilizes on the surface of azide-terminal polycaprolactone microcarriers within 10 min compared to 12 h for other conjugation techniques, and it is conducted in physiological conditions and in the absence of coupling reagents.
Identifiants
pubmed: 30730698
doi: 10.1021/acs.bioconjchem.8b00868
doi:
Substances chimiques
Alkynes
0
Azides
0
Cyclooctanes
0
Emulsions
0
Immobilized Proteins
0
Polyesters
0
polycaprolactone
24980-41-4
Serum Albumin, Human
ZIF514RVZR
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM