Mitotic chromosome alignment ensures mitotic fidelity by promoting interchromosomal compaction during anaphase.
Journal
The Journal of cell biology
ISSN: 1540-8140
Titre abrégé: J Cell Biol
Pays: United States
ID NLM: 0375356
Informations de publication
Date de publication:
01 04 2019
01 04 2019
Historique:
received:
31
07
2018
revised:
21
11
2018
accepted:
09
01
2019
pubmed:
9
2
2019
medline:
14
4
2020
entrez:
9
2
2019
Statut:
ppublish
Résumé
Chromosome alignment at the equator of the mitotic spindle is a highly conserved step during cell division; however, its importance to genomic stability and cellular fitness is not understood. Normal mammalian somatic cells lacking KIF18A function complete cell division without aligning chromosomes. These alignment-deficient cells display normal chromosome copy numbers in vitro and in vivo, suggesting that chromosome alignment is largely dispensable for maintenance of euploidy. However, we find that loss of chromosome alignment leads to interchromosomal compaction defects during anaphase, abnormal organization of chromosomes into a single nucleus at mitotic exit, and the formation of micronuclei in vitro and in vivo. These defects slow cell proliferation and are associated with impaired postnatal growth and survival in mice. Our studies support a model in which the alignment of mitotic chromosomes promotes proper organization of chromosomes into a single nucleus and continued proliferation by ensuring that chromosomes segregate as a compact mass during anaphase.
Identifiants
pubmed: 30733233
pii: jcb.201807228
doi: 10.1083/jcb.201807228
pmc: PMC6446859
doi:
Substances chimiques
KIF18a protein, mouse
0
TP53 protein, human
0
Tumor Suppressor Protein p53
0
KIF18A protein, human
EC 3.6.1.-
Kinesins
EC 3.6.4.4
Banques de données
GENBANK
['BC048347']
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, Non-P.H.S.
Video-Audio Media
Langues
eng
Sous-ensembles de citation
IM
Pagination
1148-1163Subventions
Organisme : NIGMS NIH HHS
ID : R01 GM086610
Pays : United States
Organisme : NIGMS NIH HHS
ID : R01 GM121491
Pays : United States
Organisme : NICHD NIH HHS
ID : R03 HD078485
Pays : United States
Organisme : NIH HHS
ID : S10 OD018175
Pays : United States
Commentaires et corrections
Type : CommentIn
Informations de copyright
© 2019 Fonseca et al.
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