Endophilin-A regulates presynaptic Ca


Journal

The EMBO journal
ISSN: 1460-2075
Titre abrégé: EMBO J
Pays: England
ID NLM: 8208664

Informations de publication

Date de publication:
01 03 2019
Historique:
received: 22 06 2018
revised: 17 12 2018
accepted: 20 12 2018
pubmed: 9 2 2019
medline: 7 1 2020
entrez: 9 2 2019
Statut: ppublish

Résumé

Ribbon synapses of cochlear inner hair cells (IHCs) operate with high rates of neurotransmission; yet, the molecular regulation of synaptic vesicle (SV) recycling at these synapses remains poorly understood. Here, we studied the role of endophilins-A1-3, endocytic adaptors with curvature-sensing and curvature-generating properties, in mouse IHCs. Single-cell RT-PCR indicated the expression of endophilins-A1-3 in IHCs, and immunoblotting confirmed the presence of endophilin-A1 and endophilin-A2 in the cochlea. Patch-clamp recordings from endophilin-A-deficient IHCs revealed a reduction of Ca

Identifiants

pubmed: 30733243
pii: embj.2018100116
doi: 10.15252/embj.2018100116
pmc: PMC6396150
pii:
doi:

Substances chimiques

Membrane Proteins 0
otoferlin protein, mouse 0
Acyltransferases EC 2.3.-
2-acylglycerophosphate acyltransferase EC 2.3.1.52
Calcium SY7Q814VUP

Types de publication

Journal Article Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Informations de copyright

© 2019 The Authors.

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Auteurs

Jana Kroll (J)

Synaptic Vesicle Dynamics Group, European Neuroscience Institute (ENI), University Medical Center Göttingen, Göttingen, Germany.
Molecular Architecture of Synapses Group, Institute for Auditory Neuroscience, InnerEarLab and Center for Biostructural Imaging of Neurodegeneration, University Medical Center Göttingen, Göttingen, Germany.
Collaborative Research Center 889, University of Göttingen, Göttingen, Germany.
Göttingen Graduate School for Neuroscience and Molecular Biosciences, University of Göttingen, Göttingen, Germany.

Lina M Jaime Tobón (LM)

Collaborative Research Center 889, University of Göttingen, Göttingen, Germany.
Göttingen Graduate School for Neuroscience and Molecular Biosciences, University of Göttingen, Göttingen, Germany.
Institute for Auditory Neuroscience and InnerEarLab, University Medical Center Göttingen, Göttingen, Germany.
Synaptic Nanophysiology Group, Max Planck Institute for Biophysical Chemistry, Göttingen, Germany.
Auditory Neuroscience Group, Max Planck Institute for Experimental Medicine, Göttingen, Germany.

Christian Vogl (C)

Collaborative Research Center 889, University of Göttingen, Göttingen, Germany.
Institute for Auditory Neuroscience and InnerEarLab, University Medical Center Göttingen, Göttingen, Germany.
Auditory Neuroscience Group, Max Planck Institute for Experimental Medicine, Göttingen, Germany.
Presynaptogenesis and Intracellular Transport in Hair Cells Group, Institute for Auditory Neuroscience and InnerEarLab, University Medical Center Göttingen, Göttingen, Germany.

Jakob Neef (J)

Collaborative Research Center 889, University of Göttingen, Göttingen, Germany.
Institute for Auditory Neuroscience and InnerEarLab, University Medical Center Göttingen, Göttingen, Germany.
Synaptic Nanophysiology Group, Max Planck Institute for Biophysical Chemistry, Göttingen, Germany.
Auditory Neuroscience Group, Max Planck Institute for Experimental Medicine, Göttingen, Germany.

Ilona Kondratiuk (I)

Synaptic Vesicle Dynamics Group, European Neuroscience Institute (ENI), University Medical Center Göttingen, Göttingen, Germany.

Melanie König (M)

Synaptic Vesicle Dynamics Group, European Neuroscience Institute (ENI), University Medical Center Göttingen, Göttingen, Germany.
Collaborative Research Center 889, University of Göttingen, Göttingen, Germany.

Nicola Strenzke (N)

Collaborative Research Center 889, University of Göttingen, Göttingen, Germany.
Auditory Neuroscience Group, Max Planck Institute for Experimental Medicine, Göttingen, Germany.
Auditory Systems Physiology Group and InnerEarLab, Department of Otolaryngology, University of Göttingen Medical Center, Göttingen, Germany.

Carolin Wichmann (C)

Molecular Architecture of Synapses Group, Institute for Auditory Neuroscience, InnerEarLab and Center for Biostructural Imaging of Neurodegeneration, University Medical Center Göttingen, Göttingen, Germany carolin.wichmann@med.uni-goettingen.de i.milosevic@eni-g.de tmoser@gwdg.de.
Collaborative Research Center 889, University of Göttingen, Göttingen, Germany.
Auditory Neuroscience Group, Max Planck Institute for Experimental Medicine, Göttingen, Germany.

Ira Milosevic (I)

Synaptic Vesicle Dynamics Group, European Neuroscience Institute (ENI), University Medical Center Göttingen, Göttingen, Germany carolin.wichmann@med.uni-goettingen.de i.milosevic@eni-g.de tmoser@gwdg.de.
Collaborative Research Center 889, University of Göttingen, Göttingen, Germany.

Tobias Moser (T)

Collaborative Research Center 889, University of Göttingen, Göttingen, Germany carolin.wichmann@med.uni-goettingen.de i.milosevic@eni-g.de tmoser@gwdg.de.
Institute for Auditory Neuroscience and InnerEarLab, University Medical Center Göttingen, Göttingen, Germany.
Synaptic Nanophysiology Group, Max Planck Institute for Biophysical Chemistry, Göttingen, Germany.
Auditory Neuroscience Group, Max Planck Institute for Experimental Medicine, Göttingen, Germany.

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Classifications MeSH