Single stranded (ss)RNA-mediated antiviral response against infectious laryngotracheitis virus infection.
Chicken
Infectious laryngotracheitis virus
Interleukin 1β
Macrophage
Nitric oxide
Resiquimod
Type 1 interferon
ssRNA
Journal
BMC microbiology
ISSN: 1471-2180
Titre abrégé: BMC Microbiol
Pays: England
ID NLM: 100966981
Informations de publication
Date de publication:
08 02 2019
08 02 2019
Historique:
received:
11
05
2018
accepted:
23
01
2019
entrez:
10
2
2019
pubmed:
10
2
2019
medline:
18
12
2019
Statut:
epublish
Résumé
Single stranded ribonucleic acid (ssRNA) binds to toll-like receptor (TLR)7 leading to recruitment of immune cells and production of pro-inflammatory cytokines, which has been shown in mammals. In chickens, synthetic ssRNA analog, resiquimod, has been shown to elicit antiviral response against infectious bursal disease virus infection. The objective of this study was to determine the innate host responses activated by the pre-hatch in ovo administration of resiquimod against infectious laryngotracheitis virus (ILTV) infection in chickens post-hatch. First, we observed that in ovo treatment of resiquimod at embryo day (ED) 18 increases macrophage recruitment in respiratory and gastrointestinal tissues of chicken day 1 post-hatch in addition to interleukin (IL)-1β in lungs. Second, we observed that in ovo treatment of resiquimod reduces ILTV cloacal shedding at 7 days post-infection (dpi) when challenged at day 1 post-hatch coinciding with higher macrophage recruitment. In vitro, we found that resiquimod enhances production of nitric oxide (NO) and IL-1β and not type 1 interferon (IFN) activity in avian macrophages. Although, the antiviral response against ILTV is associated with the enhanced innate immune response, it is not dependent on any of the innate immune mediators observed as has been shown in vitro using avian macrophage. This study provides insights into the mechanisms of antiviral response mediated by resiquimod, particularly against ILTV infection in chicken.
Sections du résumé
BACKGROUND
Single stranded ribonucleic acid (ssRNA) binds to toll-like receptor (TLR)7 leading to recruitment of immune cells and production of pro-inflammatory cytokines, which has been shown in mammals. In chickens, synthetic ssRNA analog, resiquimod, has been shown to elicit antiviral response against infectious bursal disease virus infection. The objective of this study was to determine the innate host responses activated by the pre-hatch in ovo administration of resiquimod against infectious laryngotracheitis virus (ILTV) infection in chickens post-hatch.
RESULTS
First, we observed that in ovo treatment of resiquimod at embryo day (ED) 18 increases macrophage recruitment in respiratory and gastrointestinal tissues of chicken day 1 post-hatch in addition to interleukin (IL)-1β in lungs. Second, we observed that in ovo treatment of resiquimod reduces ILTV cloacal shedding at 7 days post-infection (dpi) when challenged at day 1 post-hatch coinciding with higher macrophage recruitment. In vitro, we found that resiquimod enhances production of nitric oxide (NO) and IL-1β and not type 1 interferon (IFN) activity in avian macrophages. Although, the antiviral response against ILTV is associated with the enhanced innate immune response, it is not dependent on any of the innate immune mediators observed as has been shown in vitro using avian macrophage.
CONCLUSION
This study provides insights into the mechanisms of antiviral response mediated by resiquimod, particularly against ILTV infection in chicken.
Identifiants
pubmed: 30736730
doi: 10.1186/s12866-019-1398-6
pii: 10.1186/s12866-019-1398-6
pmc: PMC6368756
doi:
Substances chimiques
Antiviral Agents
0
Cytokines
0
Imidazoles
0
RNA
63231-63-0
resiquimod
V3DMU7PVXF
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
34Références
J Gen Virol. 2017 Jun;98(6):1209-1223
pubmed: 28613150
Annu Rev Immunol. 1997;15:323-50
pubmed: 9143691
PLoS One. 2018 Mar 7;13(3):e0193964
pubmed: 29513732
Immunology. 2006 Jan;117(1):11-21
pubmed: 16423036
J Immunol. 2006 May 1;176(9):5205-12
pubmed: 16621985
PLoS Pathog. 2012;8(11):e1003039
pubmed: 23209411
J Mol Biol. 2014 Mar 20;426(6):1246-64
pubmed: 24316048
Res Vet Sci. 2015 Jun;100:105-8
pubmed: 25935758
Viruses. 2015 Apr 08;7(4):1832-52
pubmed: 25856635
J Interferon Cytokine Res. 2012 Jan;32(1):46-51
pubmed: 21929369
J Immunol. 2006 Sep 1;177(5):3337-43
pubmed: 16920974
Blood. 1999 Jul 15;94(2):572-8
pubmed: 10397724
Bioorg Med Chem. 2008 Jan 15;16(2):658-67
pubmed: 17988874
Arch Virol. 2016 Mar;161(3):521-8
pubmed: 26597188
Proc Natl Acad Sci U S A. 2003 Sep 16;100(19):10890-5
pubmed: 12960395
Avian Dis. 2001 Apr-Jun;45(2):389-99
pubmed: 11417818
Nature. 2006 Jul 6;442(7098):39-44
pubmed: 16823444
Vet Immunol Immunopathol. 2015 Apr 15;164(3-4):170-8
pubmed: 25764942
Tissue Antigens. 2006 Apr;67(4):267-76
pubmed: 16634862
Avian Dis. 2003 Oct-Dec;47(4):1423-8
pubmed: 14708991
J Interferon Cytokine Res. 2001 Dec;21(12):1071-7
pubmed: 11798465
J Biol Chem. 2007 May 25;282(21):15319-23
pubmed: 17395581
Oncotarget. 2017 Aug 7;8(41):70847-70853
pubmed: 29050325
Int Rev Immunol. 2013 Apr;32(2):116-33
pubmed: 23570313
Dev Comp Immunol. 2015 Jan;48(1):22-32
pubmed: 25195716
Virology. 1998 Sep 1;248(2):295-304
pubmed: 9721238
J Virol. 2000 Apr;74(8):3605-12
pubmed: 10729136
J Innate Immun. 2010;2(1):56-65
pubmed: 20375623
J Interferon Cytokine Res. 2011 Jul;31(7):533-8
pubmed: 21323426
Arch Virol. 2016 Aug;161(8):2075-86
pubmed: 27233799
J Vet Med Sci. 2011 Feb;73(2):209-15
pubmed: 20948168
J Virol. 2000 Apr;74(7):3399-403
pubmed: 10708457
Cell. 1997 Oct 31;91(3):295-8
pubmed: 9363937
J Biol Chem. 2003 Oct 3;278(40):38105-8
pubmed: 12893815
Nat Commun. 2011 Feb 08;2:190
pubmed: 21304519
Vet Microbiol. 2016 May 1;187:21-30
pubmed: 27066705
Immunology. 2005 Apr;114(4):507-21
pubmed: 15804288
Avian Dis. 1996 Apr-Jun;40(2):342-8
pubmed: 8790884
Dev Comp Immunol. 2011 Jan;35(1):28-34
pubmed: 20692289
Arch Virol. 2014 Nov;159(11):2937-48
pubmed: 24934575
J Interferon Cytokine Res. 2011 Jun;31(6):515-25
pubmed: 21323570
Antiviral Res. 2016 Jun;130:36-45
pubmed: 26971407
PLoS One. 2017 Jan 3;12(1):e0169154
pubmed: 28045984
Front Immunol. 2018 Jun 13;9:1368
pubmed: 29951070
J Interferon Cytokine Res. 2001 May;21(5):287-96
pubmed: 11429159
Avian Dis. 2002 Apr-Jun;46(2):274-80
pubmed: 12061635
Innate Immun. 2012 Feb;18(1):179-89
pubmed: 21239454
Vet Microbiol. 2018 Feb;215:1-10
pubmed: 29426399
J Virol. 2001 Nov;75(22):10730-7
pubmed: 11602714
Inflamm Res. 2011 Mar;60(3):271-9
pubmed: 20953969
Vet Immunol Immunopathol. 2013 Oct 1;155(4):270-5
pubmed: 24034933
Poult Sci. 2012 Apr;91(4):1038-42
pubmed: 22399745
Virol J. 2018 Mar 12;15(1):43
pubmed: 29530062
Annu Rev Immunol. 1984;2:395-428
pubmed: 6242349
Vet Microbiol. 2007 Apr 15;121(3-4):278-87
pubmed: 17254721
Vet Immunol Immunopathol. 1990 Nov;26(3):237-50
pubmed: 2176014
Vet Immunol Immunopathol. 2012 May 15;146(3-4):212-24
pubmed: 22475571
J Virol Methods. 2018 Feb;252:1-7
pubmed: 28834736
Curr Opin Virol. 2011 Dec;1(6):447-54
pubmed: 22440908
Viral Immunol. 2018 Jul/Aug;31(6):433-446
pubmed: 29813000
Biol Reprod. 2014 Jan 23;90(1):11
pubmed: 24285719