Equilibrium Modeling of the Mechanics and Structure of the Cancer Glycocalyx.
Journal
Biophysical journal
ISSN: 1542-0086
Titre abrégé: Biophys J
Pays: United States
ID NLM: 0370626
Informations de publication
Date de publication:
19 02 2019
19 02 2019
Historique:
received:
05
10
2018
accepted:
28
12
2018
pubmed:
10
2
2019
medline:
22
1
2020
entrez:
10
2
2019
Statut:
ppublish
Résumé
The glycocalyx is a thick coat of proteins and carbohydrates on the outer surface of all eukaryotic cells. Overproduction of large, flexible or rod-like biopolymers, including hyaluronic acid and mucins, in the glycocalyx strongly correlates with the aggression of many cancer types. However, theoretical frameworks to predict the effects of these changes on cancer cell adhesion and other biophysical processes remain limited. Here, we propose a detailed modeling framework for the glycocalyx incorporating important physical effects of biopolymer flexibility, excluded volume, counterion mobility, and coupled membrane deformations. Because mucin and hyaluronic biopolymers are proposed to extend and rigidify depending on the extent of their decoration with side chains, we propose and consider two limiting cases for the structural elements of the glycocalyx: stiff beams and flexible chains. Simulations predict the mechanical response of the glycocalyx to compressive loads, which are imposed on cells residing in the highly confined spaces of the solid tumor or invaded tissues. Notably, the shape of the mechanical response transitions from hyperbolic to sigmoidal for more rod-like glycocalyx elements. These mechanical responses, along with the corresponding equilibrium protein organizations and membrane topographies, are summarized to aid in hypothesis generation and the evaluation of future experimental measurements. Overall, the modeling framework developed provides a theoretical basis for understanding the physical biology of the glycocalyx in human health.
Identifiants
pubmed: 30736980
pii: S0006-3495(19)30030-X
doi: 10.1016/j.bpj.2018.12.023
pmc: PMC6382957
pii:
doi:
Substances chimiques
Glycoproteins
0
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Langues
eng
Sous-ensembles de citation
IM
Pagination
694-708Subventions
Organisme : NIGMS NIH HHS
ID : DP2 GM119133
Pays : United States
Organisme : NCI NIH HHS
ID : U54 CA210184
Pays : United States
Informations de copyright
Copyright © 2019 Biophysical Society. Published by Elsevier Inc. All rights reserved.
Références
Science. 2016 Apr 15;352(6283):353-8
pubmed: 27013428
Biophys J. 2002 Sep;83(3):1661-70
pubmed: 12202389
Eur Phys J E Soft Matter. 2006 Jul;20(3):243-56
pubmed: 16862399
J Cell Sci. 2000 May;113 ( Pt 9):1589-600
pubmed: 10751150
Soft Matter. 2016 Feb 21;12(7):2127-34
pubmed: 26751985
PLoS One. 2011 Mar 29;6(3):e17833
pubmed: 21468318
Proc Natl Acad Sci U S A. 2013 Mar 19;110(12):4534-8
pubmed: 23471983
Biophys J. 2009 May 6;96(9):3555-72
pubmed: 19413961
Phys Rev A Gen Phys. 1987 Oct 15;36(8):3912-3925
pubmed: 9899329
J Cell Biol. 2010 Jan 11;188(1):11-9
pubmed: 19951899
Q Rev Biophys. 2011 Nov;44(4):391-432
pubmed: 21729348
Biophys J. 1976 Aug;16(8):861-8
pubmed: 938726
J Cell Sci. 2004 Nov 1;117(Pt 23):5521-34
pubmed: 15479718
Cell. 2015 Feb 12;160(4):659-672
pubmed: 25679760
J Cell Biol. 2009 Jun 29;185(7):1275-84
pubmed: 19564406
Chembiochem. 2004 Oct 4;5(10):1393-9
pubmed: 15457530
Biophys J. 1983 Jul;43(1):27-30
pubmed: 6882860
J Theor Biol. 1970 Jan;26(1):61-81
pubmed: 5411112
Nat Rev Gastroenterol Hepatol. 2013 Oct;10(10):607-20
pubmed: 23856888
Nat Rev Cancer. 2009 Dec;9(12):874-85
pubmed: 19935676
Proc Natl Acad Sci U S A. 2015 Oct 13;112(41):12574-9
pubmed: 26420872
Soft Matter. 2015 May 14;11(18):3693-705
pubmed: 25823723
Sci Rep. 2015 Jul 21;5:12314
pubmed: 26197304
Annu Rev Biophys Biomol Struct. 2005;34:351-78
pubmed: 15869394
Dev Cell. 2010 Aug 17;19(2):194-206
pubmed: 20708583
Br J Exp Pathol. 1985 Oct;66(5):543-9
pubmed: 4063159
Biophys J. 2016 Jun 21;110(12):2739-2750
pubmed: 27332132
Phys Rev Lett. 2004 Dec 17;93(25):256001
pubmed: 15697914
Proc Natl Acad Sci U S A. 1991 Oct 1;88(19):8392-6
pubmed: 1717976
Nat Chem Biol. 2015 Nov;11(11):822-5
pubmed: 26485070
Cell Biophys. 1992 Apr-Jun;20(2-3):177-222
pubmed: 1285299
J Cell Biol. 2002 Jun 10;157(6):1071-81
pubmed: 12058021
Proc Natl Acad Sci U S A. 2003 Jun 24;100(13):7988-95
pubmed: 12810946
Cancer Res. 2016 May 1;76(9):2507-12
pubmed: 27197262
Curr Opin Cell Biol. 2011 Aug;23(4):393-403
pubmed: 21474295
Clin Chem. 1995 Oct;41(10):1522-5
pubmed: 7586528
ACS Biomater Sci Eng. 2018 Feb 12;4(2):388-399
pubmed: 29805991
J Phys Chem B. 2009 Jun 11;113(23):7995-8005
pubmed: 19323472
J Biomech. 2009 Feb 9;42(3):303-7
pubmed: 19144337
J Mammary Gland Biol Neoplasia. 2001 Jul;6(3):339-53
pubmed: 11547902
Sci Adv. 2016 Nov 11;2(11):e1601478
pubmed: 28861466
Nat Biotechnol. 1997 Aug;15(8):778-83
pubmed: 9255794
Trends Cell Biol. 2015 Nov;25(11):666-674
pubmed: 26437597
Nature. 2014 Jul 17;511(7509):319-25
pubmed: 25030168
Science. 2002 May 17;296(5571):1308-13
pubmed: 11964443
Adv Cancer Res. 2014;123:1-34
pubmed: 25081524
Proc Natl Acad Sci U S A. 2006 Jul 5;103(27):10259-10264
pubmed: 16793927
Biomaterials. 1997 Aug;18(16):1091-8
pubmed: 9247346
Biophys J. 1980 May;30(2):265-84
pubmed: 7260275
PLoS Comput Biol. 2009 Dec;5(12):e1000604
pubmed: 20011123
Nat Phys. 2018;14(7):658-669
pubmed: 33859716
Science. 1991 Feb 22;251(4996):905-14
pubmed: 17847384
Z Naturforsch C. 1973 Nov-Dec;28(11):693-703
pubmed: 4273690
Phys Rev Lett. 1990 Apr 23;64(17):2094-2097
pubmed: 10041575
Proc Natl Acad Sci U S A. 2016 Jul 19;113(29):8177-81
pubmed: 27382181
Nat Rev Mol Cell Biol. 2014 Dec;15(12):813-24
pubmed: 25355506
Front Oncol. 2017 Oct 09;7:242
pubmed: 29062810
Nat Struct Mol Biol. 2010 Jun;17(6):659-65
pubmed: 20495561
Arch Biochem Biophys. 1977 Apr 15;180(1):57-68
pubmed: 856045
Proc Natl Acad Sci U S A. 2009 Jun 23;106(25):10097-102
pubmed: 19541627
Nat Rev Cancer. 2005 Jan;5(1):29-41
pubmed: 15630413
Biomacromolecules. 2003 Nov-Dec;4(6):1805-10
pubmed: 14606912
J Cell Sci. 1986 Dec;86:249-61
pubmed: 2443520
Proc Natl Acad Sci U S A. 2002 May 28;99(11):7426-31
pubmed: 12032299
J Struct Biol. 2001 Dec;136(3):239-55
pubmed: 12051903
Curr Opin Cell Biol. 2010 Aug;22(4):430-6
pubmed: 20605711
J Chem Phys. 2006 Feb 21;124(7):74903
pubmed: 16497078
Nat Commun. 2014 Aug 20;5:4683
pubmed: 25140641