Permeability of the porcine iris stroma.

Animals Cell Membrane Permeability / physiology Iris / cytology Models, Animal Stromal Cells / cytology Swine Tomography, Optical Coherence
Angle closure glaucoma Aqueous humor Biphasic tissue Dilator muscles Hydraulic permeability Iris biomechanics Iris pigment epithelium Sphincter muscles

Journal

Experimental eye research
ISSN: 1096-0007
Titre abrégé: Exp Eye Res
Pays: England
ID NLM: 0370707

Informations de publication

Date de publication:
04 2019
Historique:
received: 08 11 2018
revised: 11 01 2019
accepted: 04 02 2019
pubmed: 10 2 2019
medline: 14 2 2020
entrez: 10 2 2019
Statut: ppublish

Résumé

Current literature has not considered or provided any data on the permeability of the iris stroma. In this study, we aimed to determine the hydraulic permeability of porcine irides from the isolated stroma. Fifteen enucleated porcine eyes were acquired from the local abattoir. The iris pigment epithelium was scraped off using a pair of forceps and the dilator muscles were pinched off using a pair of colibri toothed forceps. We designed an experimental setup, based on Darcy's law, and consisting of a custom 3D-printed pressure column using acrylonitrile butadiene styrene (ABS) plastic. PBS solution was passed through the iris stroma in a 180° arc shape, with a column height of approximately 204 mm (2000 Pa). Measurements of iris stromal thickness were conducted using optical coherence tomography (OCT). To measure flow rate, we measured the mass (volume) of PBS solution using a mass balance in approximately 1 min. Histology was performed using hematoxylin and eosin (H&E) and anti-smooth muscle antibody (anti-α-SMA) for validation. The permeability experiments demonstrated that the iris stroma is a biphasic tissue that allows fluid flow. Our image processing results determined the area of flow to be 7.55 mm

Identifiants

DOI: 10.1016/j.exer.2019.02.005 PMID: 30738068
pubmed: 30738068
pii: S0014-4835(18)30788-7
doi: 10.1016/j.exer.2019.02.005
pii:
doi:

Types de publication

Journal Article Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Pagination

190-196

Informations de copyright

Copyright © 2019 Elsevier Ltd. All rights reserved.

Auteurs

Royston K Y Tan (RKY)

Ophthalmic Engineering & Innovation Laboratory, Department of Biomedical Engineering, Faculty of Engineering, National University of Singapore, Singapore.

Xiaofei Wang (X)

Ophthalmic Engineering & Innovation Laboratory, Department of Biomedical Engineering, Faculty of Engineering, National University of Singapore, Singapore; Beijing Advanced Innovation Center for Biomedical Engineering, School of Biological Science and Medical Engineering, Beihang University, Beijing, China.

Anita S Y Chan (ASY)

Singapore Eye Research Institute, Singapore National Eye Centre, Singapore; Duke-NUS Medical School, Singapore.

Monisha Esther Nongpiur (ME)

Singapore Eye Research Institute, Singapore National Eye Centre, Singapore.

Craig Boote (C)

Ophthalmic Engineering & Innovation Laboratory, Department of Biomedical Engineering, Faculty of Engineering, National University of Singapore, Singapore; Structural Biophysics Group, School of Optometry and Vision Sciences, Cardiff University, UK.

Shamira A Perera (SA)

Singapore Eye Research Institute, Singapore National Eye Centre, Singapore; Duke-NUS Medical School, Singapore.

Michaël J A Girard (MJA)

Ophthalmic Engineering & Innovation Laboratory, Department of Biomedical Engineering, Faculty of Engineering, National University of Singapore, Singapore; Singapore Eye Research Institute, Singapore National Eye Centre, Singapore. Electronic address: mgirard@nus.edu.sg.

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Classifications MeSH

questionsmedicales.fr › Eucaryotes › Animaux › Permeability of the porcine iris stroma.
questionsmedicales.fr › Phénomènes physiologiques cellulaires › Perméabilité des membranes cellulaires › Permeability of the porcine iris stroma.
questionsmedicales.fr › Organes des sens › Oeil › Uvée › Iris › Permeability of the porcine iris stroma.
questionsmedicales.fr › Techniques d'investigation › Modèles animaux › Permeability of the porcine iris stroma.
questionsmedicales.fr › Cellules › Cellules du tissu conjonctif › Cellules stromales › Permeability of the porcine iris stroma.
questionsmedicales.fr › Eucaryotes › Animaux › Chordés › Vertébrés › Mammifères › Eutheria › Artiodactyla › Suidae › Permeability of the porcine iris stroma.
questionsmedicales.fr › Techniques d'investigation › Imagerie optique › Tomographie optique › Tomographie par cohérence optique › Permeability of the porcine iris stroma.