Comparison of FTD® respiratory pathogens 33 and a singleplex CDC assay for the detection of respiratory viruses: A study from Cameroon.
Adolescent
Adult
Aged
Aged, 80 and over
Cameroon
Child
Child, Preschool
Clinical Laboratory Techniques
/ methods
Diagnostic Tests, Routine
/ methods
Female
Humans
Infant
Infant, Newborn
Male
Middle Aged
Molecular Diagnostic Techniques
/ methods
Nasopharynx
/ virology
Real-Time Polymerase Chain Reaction
/ methods
Respiratory Tract Infections
/ diagnosis
Reverse Transcriptase Polymerase Chain Reaction
/ methods
Virus Diseases
/ diagnosis
Cameroon
Molecular diagnostic
Respiratory viruses
Journal
Diagnostic microbiology and infectious disease
ISSN: 1879-0070
Titre abrégé: Diagn Microbiol Infect Dis
Pays: United States
ID NLM: 8305899
Informations de publication
Date de publication:
Jul 2019
Jul 2019
Historique:
received:
03
10
2018
revised:
09
01
2019
accepted:
09
01
2019
pubmed:
11
2
2019
medline:
8
11
2019
entrez:
11
2
2019
Statut:
ppublish
Résumé
This study compares the detection of 14 common respiratory viruses by two different real-time reverse transcription polymerase chain reaction (rRT-PCR) methods: in house singleplex tests developed by the Centers for Disease Control and Prevention and the commercially available Fast Track Diagnostic (FTD®) Respiratory Pathogens 33 multiplex test. A total of 217 nasopharyngeal swabs were analyzed using CDC singleplex rRT-PCR and FTD® Respiratory Pathogens 33 multiplex assays, for the detection of 14 respiratory viruses. The results showed that 179/217 (82.5%) samples were positive with the singleplex method and 183/217 (84.3%) with the FTD® Respiratory Pathogens 33 multiplex test. Excellent or satisfactory agreement was obtained for all viruses (k > 0.6) except Parainfluenzavirus 4 (k = 0.24) and influenza B (k = 0.51). Although the multiplex FTD kits were more expensive than the singleplex assay, the FTD kits yielded rapid results in a shorter timeframe, increasing efficiency of diagnosis.
Identifiants
pubmed: 30738690
pii: S0732-8893(18)30448-6
doi: 10.1016/j.diagmicrobio.2019.01.007
pmc: PMC7127211
pii:
doi:
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
236-242Informations de copyright
Copyright © 2019 Elsevier Inc. All rights reserved.
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