Ultrasound-responsive nanobubble-mediated gene transfection in the cerebroventricular region by intracerebroventricular administration in mice.

Animals Brain / metabolism DNA / administration & dosage Gene Transfer Techniques Injections, Intraventricular Male Mice Nanostructures Plasmids / administration & dosage Transfection / methods Ultrasonic Waves
Choroid plexus Gene delivery Intracerebroventricular injection Nanobubbles Ultrasound plasmid DNA

Journal

European journal of pharmaceutics and biopharmaceutics : official journal of Arbeitsgemeinschaft fur Pharmazeutische Verfahrenstechnik e.V
ISSN: 1873-3441
Titre abrégé: Eur J Pharm Biopharm
Pays: Netherlands
ID NLM: 9109778

Informations de publication

Date de publication:
Apr 2019
Historique:
received: 14 11 2018
revised: 05 02 2019
accepted: 06 02 2019
pubmed: 11 2 2019
medline: 14 6 2019
entrez: 11 2 2019
Statut: ppublish

Résumé

Intracerebroventricular (ICV) administration of ultrasound-responsive bubbles and cranial ultrasound irradiation is reported as a transfection system for the cerebroventricular region. This study aimed to characterize the transfection system with respect to transfection efficiency, spatial distribution of transgene expression, and safety. Plasmid DNA was transfected to mouse brain by ICV injection of ultrasound-responsive nanobubbles, followed by ultrasound irradiation to brain. Spatial distribution of transgene expression in the cerebroventricular region was investigated using multicolor deep imaging. This transfection system efficiently transferred the transgene to the choroid plexus with no morphological change or cerebral hemorrhage. Moreover, sustained secretion of transgenic protein was achieved by transferring the transgene encoding the secretable protein. We successfully developed an ultrasound-responsive nanobubbles-mediated method for gene transfection into the cerebroventricular region via ICV administration in mice.

Identifiants

DOI: 10.1016/j.ejpb.2019.02.003 PMID: 30738859
pubmed: 30738859
pii: S0939-6411(18)31414-0
doi: 10.1016/j.ejpb.2019.02.003
pii:
doi:

Substances chimiques

DNA 9007-49-2

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Pagination

1-8

Informations de copyright

Copyright © 2019. Published by Elsevier B.V.

Auteurs

Koki Ogawa (K)

Graduate School of Biomedical Sciences, Nagasaki University, 1-7-1 Sakamoto, Nagasaki-shi, Nagasaki 852-8588, Japan.

Yuki Fuchigami (Y)

Graduate School of Biomedical Sciences, Nagasaki University, 1-7-1 Sakamoto, Nagasaki-shi, Nagasaki 852-8588, Japan. Electronic address: y-fuchigami@nagasaki-u.ac.jp.

Masayori Hagimori (M)

Graduate School of Biomedical Sciences, Nagasaki University, 1-7-1 Sakamoto, Nagasaki-shi, Nagasaki 852-8588, Japan. Electronic address: hagimori@nagasaki-u.ac.jp.

Shintaro Fumoto (S)

Graduate School of Biomedical Sciences, Nagasaki University, 1-7-1 Sakamoto, Nagasaki-shi, Nagasaki 852-8588, Japan. Electronic address: sfumoto@nagasaki-u.ac.jp.

Kazuo Maruyama (K)

Faculty of Pharma-Sciences, Teikyo University, 2-11-1 Kaga, Itabashiku, Tokyo 173-8605, Japan. Electronic address: maruyama@pharm.teikyo-u.ac.jp.

Shigeru Kawakami (S)

Graduate School of Biomedical Sciences, Nagasaki University, 1-7-1 Sakamoto, Nagasaki-shi, Nagasaki 852-8588, Japan. Electronic address: skawakam@nagasaki-u.ac.jp.

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Classifications MeSH

questionsmedicales.fr › Eucaryotes › Animaux › Ultrasound-responsive nanobubble-mediated gene...
questionsmedicales.fr › Système nerveux › Système nerveux central › Encéphale › Ultrasound-responsive nanobubble-mediated gene...
questionsmedicales.fr › Acides nucléiques, nucléotides et nucléosides › Acides nucléiques › ADN › Ultrasound-responsive nanobubble-mediated gene...
questionsmedicales.fr › Techniques d'investigation › Techniques génétiques › Techniques de transfert de gènes › Ultrasound-responsive nanobubble-mediated gene...
questionsmedicales.fr › Thérapeutique › Traitement médicamenteux › Voies d'administration de substances chimiques et des médicaments › Injections › Injections ventriculaires › Ultrasound-responsive nanobubble-mediated gene...
questionsmedicales.fr › Eucaryotes › Animaux › Chordés › Vertébrés › Mammifères › Eutheria › Rodentia › Muridae › Murinae › Souris › Ultrasound-responsive nanobubble-mediated gene...
questionsmedicales.fr › Technologie, industrie et agriculture › Produits manufacturés › Nanostructures › Ultrasound-responsive nanobubble-mediated gene...
questionsmedicales.fr › Phénomènes génétiques › Structures génétiques › Plasmides › Ultrasound-responsive nanobubble-mediated gene...
questionsmedicales.fr › Phénomènes génétiques › Recombinaison génétique › Transfection › Ultrasound-responsive nanobubble-mediated gene...
questionsmedicales.fr › Phénomènes physiques › Rayonnement › Rayonnement non ionisant › Son (physique) › Ondes ultrasonores › Ultrasound-responsive nanobubble-mediated gene...