Mouse Ex Vivo Kidney Culture Methods.

Animals Cell Differentiation Kidney / cytology Mesoderm / cytology Mice Organ Culture Techniques / methods Ureter / cytology
Ex vivo In vitro differentiation Kidney development Metanephric mesenchyme Microdissection Organ culture Ureteric bud

Journal

Methods in molecular biology (Clifton, N.J.)
ISSN: 1940-6029
Titre abrégé: Methods Mol Biol
Pays: United States
ID NLM: 9214969

Informations de publication

Date de publication:
2019
Historique:
entrez: 12 2 2019
pubmed: 12 2 2019
medline: 21 6 2019
Statut: ppublish

Résumé

Kidney organogenesis has been a widely used classical model system to study inductive tissue interactions that guide differentiation of many organs. The basis for this is in the pioneering work done during the early 1950s when the conditions of how to support ex vivo growth and differentiation of developing kidneys were revealed. Importantly, culturing developing kidneys remains as an essential instrument to advance our understanding of molecular and cellular regulation of morphogenesis even today. Despite the fact that embryonic kidneys have been cultured for decades, it is not a trivial method and requires specific anatomical and developmental biology knowledge. This chapter outlines the general steps in organ culture and details the requirements for successful kidney explant differentiation.

Identifiants

DOI: 10.1007/978-1-4939-9021-4_2 PMID: 30742259
pubmed: 30742259
doi: 10.1007/978-1-4939-9021-4_2
doi:

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Pagination

23-30

Auteurs

Anneliis Ihermann-Hella (A)

GM-unit at Helsinki Institute of Life Science and Medicum, University of Helsinki, Helsinki, Finland.

Satu Kuure (S)

GM-unit at Helsinki Institute of Life Science and Medicum, Institute of Biotechnology, University of Helsinki, Helsinki, Finland. satu.kuure@helsinki.fi.

Articles similaires

Evaluating the efficacy of telesurgery with dual console SSI Mantra Surgical Robotic System: experiment on animal model and clinical trials.

Sudhir Prem Srivastava, Vishwajyoti Pascual Srivastava, Avinesh Singh et al.
1.00
Robotic Surgical Procedures Animals Humans Telemedicine Models, Animal

Odour generalisation and detection dog training.

Lyn Caldicott, Thomas W Pike, Helen E Zulch et al.
1.00
Animals Odorants Dogs Generalization, Psychological Smell

FBXO22 inhibits colitis and colorectal carcinogenesis by regulating the degradation of the S2448-phosphorylated form of mTOR.

Minle Li, Xuan Chen, Pengfei Qu et al.
1.00
Animals TOR Serine-Threonine Kinases Colorectal Neoplasms Colitis Mice

Use of organic material provided by an automatic enrichment device by weaner pigs and its influence on tail lesions.

Philipp Heseker, Jeanette Probst, Stefanie Ammer et al.
1.00
Animals Tail Swine Behavior, Animal Animal Husbandry

Classifications MeSH

questionsmedicales.fr › Eucaryotes › Animaux › Mouse Ex Vivo Kidney Culture Methods.
questionsmedicales.fr › Phénomènes physiologiques cellulaires › Différenciation cellulaire › Mouse Ex Vivo Kidney Culture Methods.
questionsmedicales.fr › Appareil urogénital › Voies urinaires › Rein › Mouse Ex Vivo Kidney Culture Methods.
questionsmedicales.fr › Structures de l'embryon › Feuillets embryonnaires › Mésoderme › Mouse Ex Vivo Kidney Culture Methods.
questionsmedicales.fr › Eucaryotes › Animaux › Chordés › Vertébrés › Mammifères › Eutheria › Rodentia › Muridae › Murinae › Souris › Mouse Ex Vivo Kidney Culture Methods.
questionsmedicales.fr › Techniques d'investigation › Techniques in vitro › Techniques de culture › Techniques de culture d'organes › Mouse Ex Vivo Kidney Culture Methods.
questionsmedicales.fr › Appareil urogénital › Voies urinaires › Uretère › Mouse Ex Vivo Kidney Culture Methods.