Method to quantify cytokines and chemokines in mouse brain tissue using Bio-Plex multiplex immunoassays.
Animals
Biological Assay
/ instrumentation
Biomarkers
/ analysis
Brain
/ immunology
Chemokines
/ analysis
Cytokines
/ analysis
Disease Models, Animal
Enzyme-Linked Immunosorbent Assay
/ instrumentation
High-Throughput Screening Assays
/ instrumentation
Humans
Malaria, Cerebral
/ diagnosis
Mice
Microspheres
Plasmodium berghei
/ immunology
Bio-Plex
Cerebral Malaria
Chemokine
Cytokine
Mouse brain
Plasmodium berghei
Journal
Methods (San Diego, Calif.)
ISSN: 1095-9130
Titre abrégé: Methods
Pays: United States
ID NLM: 9426302
Informations de publication
Date de publication:
01 04 2019
01 04 2019
Historique:
received:
10
10
2018
revised:
31
01
2019
accepted:
06
02
2019
pubmed:
12
2
2019
medline:
17
6
2020
entrez:
12
2
2019
Statut:
ppublish
Résumé
This protocol describes how to prepare mouse brain tissue for quantification of multiple inflammatory mediators using a multiplex bead-based immunoassay. It is important to have methods that allow quantification of multiple analytes from small amounts of tissue. Bio-Plex is a Luminex xMAP-based multiplex bead-based immunoassay technology that permits simultaneous analysis of up to 100 analytes from a single tissue sample. This assay has been used extensively to investigate analytes in plasma and serum samples as well as cultured and primary cells. Here, we describe a method for simultaneous analysis of 33 different inflammatory cytokines and chemokines from mouse brain tissue using the Bio-Plex Pro Mouse Chemokine Panel 33-Plex.
Identifiants
pubmed: 30742997
pii: S1046-2023(18)30234-2
doi: 10.1016/j.ymeth.2019.02.007
pmc: PMC6802274
mid: NIHMS1523252
pii:
doi:
Substances chimiques
Biomarkers
0
Chemokines
0
Cytokines
0
Types de publication
Journal Article
Research Support, N.I.H., Intramural
Langues
eng
Sous-ensembles de citation
IM
Pagination
22-26Subventions
Organisme : Intramural NIH HHS
ID : Z99 NS999999
Pays : United States
Informations de copyright
Copyright © 2019 Elsevier Inc. All rights reserved.
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