Establishment of a comprehensive and high throughput serological algorithm for Zika virus diagnostic testing.
Algorithms
Antibodies, Viral
/ blood
Cross Reactions
Dengue Virus
/ immunology
Enzyme-Linked Immunosorbent Assay
/ methods
Humans
Immunoglobulin G
/ blood
Immunoglobulin M
/ blood
Mass Screening
/ methods
Sensitivity and Specificity
Serologic Tests
/ methods
Zika Virus
/ immunology
Zika Virus Infection
/ diagnosis
Diagnostic testing
Serology
Zika virus
Journal
Diagnostic microbiology and infectious disease
ISSN: 1879-0070
Titre abrégé: Diagn Microbiol Infect Dis
Pays: United States
ID NLM: 8305899
Informations de publication
Date de publication:
Jun 2019
Jun 2019
Historique:
received:
27
09
2018
revised:
07
01
2019
accepted:
07
01
2019
pubmed:
13
2
2019
medline:
23
8
2019
entrez:
13
2
2019
Statut:
ppublish
Résumé
The previous serological algorithm for Zika virus (ZIKV) comprised screening by anti-ZIKV IgM capture ELISA (MAC-ELISA) for samples collected within 3 months postexposure or onset (MPEO). Samples positive by MAC-ELISA and samples collected beyond 3 MPEO were tested by the confirmatory plaque reduction neutralization test (PRNT), which proved laborious and time-consuming during the 2015 outbreak. Thus, we evaluated several ZIKV ELISAs to establish an anti-IgM and anti-IgG combination for use as a screening tool for all samples prior to PRNT confirmation. The MAC-ELISA or InBios-M in combination with the Euroimmun-G demonstrated sensitivities of 99.1% and 97.2%, respectively, and nonflavivirus specificity of 96.0%. Their cross-reactivities were 71.4% and 50.0%, respectively, for sera positive for Dengue virus antibodies. Due to near-perfect interrater agreement with PRNT and excellent detection of samples collected beyond 3 MPEO, these combinations were recommended as a screening protocol in a new high-throughput algorithm with special considerations for ZIKV diagnostics.
Identifiants
pubmed: 30744915
pii: S0732-8893(18)30423-1
doi: 10.1016/j.diagmicrobio.2019.01.004
pii:
doi:
Substances chimiques
Antibodies, Viral
0
Immunoglobulin G
0
Immunoglobulin M
0
Types de publication
Evaluation Study
Journal Article
Langues
eng
Pagination
140-146Informations de copyright
Crown Copyright © 2019. Published by Elsevier Inc. All rights reserved.