MemBright: A Family of Fluorescent Membrane Probes for Advanced Cellular Imaging and Neuroscience.
Animals
Brain
/ ultrastructure
Carbocyanines
/ analysis
Cell Line
Cell Membrane
/ ultrastructure
Dendritic Spines
/ ultrastructure
Fluorescent Dyes
/ analysis
HeLa Cells
Humans
Liver
/ ultrastructure
Mice, Inbred C57BL
Microscopy, Confocal
/ methods
Microscopy, Fluorescence
/ methods
Neurons
/ ultrastructure
Optical Imaging
/ methods
Rats, Sprague-Dawley
STORM
fluorescence imaging
membrane probes
nerve
neuron
spine
super-resolution
synapse
tissue imaging
two-photon
Journal
Cell chemical biology
ISSN: 2451-9448
Titre abrégé: Cell Chem Biol
Pays: United States
ID NLM: 101676030
Informations de publication
Date de publication:
18 04 2019
18 04 2019
Historique:
received:
06
07
2018
revised:
07
10
2018
accepted:
15
01
2019
pubmed:
13
2
2019
medline:
28
1
2020
entrez:
13
2
2019
Statut:
ppublish
Résumé
The proper staining of the plasma membrane (PM) is critical in bioimaging as it delimits the cell. Herein, we developed MemBright, a family of six cyanine-based fluorescent turn-on PM probes that emit from orange to near infrared when reaching the PM, and enable homogeneous and selective PM staining with excellent contrast in mono- and two-photon microscopy. These probes are compatible with long-term live-cell imaging and immunostaining. Moreover, MemBright label neurons in a brighter manner than surrounding cells, allowing identification of neurons in acute brain tissue sections and neuromuscular junctions without any use of transfection or transgenic animals. In addition, MemBright probes were used in super-resolution imaging to unravel the neck of dendritic spines. 3D multicolor dSTORM in combination with immunostaining revealed en-passant synapse displaying endogenous glutamate receptors clustered at the axonal-dendritic contact site. MemBright probes thus constitute a universal toolkit for cell biology and neuroscience biomembrane imaging with a variety of microscopy techniques. VIDEO ABSTRACT.
Identifiants
pubmed: 30745238
pii: S2451-9456(19)30031-5
doi: 10.1016/j.chembiol.2019.01.009
pii:
doi:
Substances chimiques
Carbocyanines
0
Fluorescent Dyes
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
600-614.e7Subventions
Organisme : European Research Council
ID : 648528
Pays : International
Informations de copyright
Copyright © 2019 Elsevier Ltd. All rights reserved.