Specific Detection of c-Kit Expressed on Human Cell Surface by Immunosensor Based on Surface Plasmon Resonance.

Antibodies, Monoclonal / immunology Cell Line, Tumor Gene Expression Regulation HEK293 Cells Humans Immunoassay / methods Proto-Oncogene Proteins c-kit / immunology Surface Plasmon Resonance / methods
SPR antibody c-Kit gelatin immunosensor mammalian cell

Journal

Analytical sciences : the international journal of the Japan Society for Analytical Chemistry
ISSN: 1348-2246
Titre abrégé: Anal Sci
Pays: Switzerland
ID NLM: 8511078

Informations de publication

Date de publication:
2019
Historique:
entrez: 13 2 2019
pubmed: 13 2 2019
medline: 30 5 2019
Statut: ppublish

Résumé

An immunosensor based on surface plasmon resonance was developed for detection of c-Kit expressed on a cell surface. The combination of the antibody solution modified with gelatin before immobilization to the sensor chip and its blocking with gelatin drastically decreased the nonspecific reaction. The condition may be useful for the detection of various cells by using antibody against cell surface marker including the c-Kit.

Identifiants

DOI: 10.2116/analsci.18N022 PMID: 30745512
pubmed: 30745512
doi: 10.2116/analsci.18N022
doi:

Substances chimiques

Antibodies, Monoclonal 0
Proto-Oncogene Proteins c-kit EC 2.7.10.1

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Pagination

223-225

Auteurs

Shiro Miyake (S)

Department of Food and Life Science, Azabu University.
Research & Development Division, Horiba, Ltd.

Daisuke Irikura (D)

Research & Development Division, Horiba, Ltd.

Tomomi Yamasaki (T)

Osaka Institute of Public Health.

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Classifications MeSH

questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Globulines › Sérum-globulines › Immunoglobulines › Anticorps › Anticorps monoclonaux › Specific Detection of c-Kit Expressed on Human...
questionsmedicales.fr › Cellules › Cellules cultivées › Cellules cancéreuses en culture › Lignée cellulaire tumorale › Specific Detection of c-Kit Expressed on Human...
questionsmedicales.fr › Phénomènes génétiques › Régulation de l'expression des gènes › Specific Detection of c-Kit Expressed on Human...
questionsmedicales.fr › Cellules › Cellules épithéliales › Cellules HEK293 › Specific Detection of c-Kit Expressed on Human...
questionsmedicales.fr › Eucaryotes › Animaux › Chordés › Vertébrés › Mammifères › Eutheria › Primates › Haplorhini › Catarrhini › Hominidae › Humains › Specific Detection of c-Kit Expressed on Human...
questionsmedicales.fr › Techniques d'investigation › Techniques de sonde moléculaire › Dosage immunologique › Specific Detection of c-Kit Expressed on Human...
questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Protéines tumorales › Protéines oncogènes › Protéines proto-oncogènes › Protéines proto-oncogènes c-kit › Specific Detection of c-Kit Expressed on Human...
questionsmedicales.fr › Techniques d'investigation › Techniques de sonde moléculaire › Techniques de biocapteur › Résonance plasmonique de surface › Specific Detection of c-Kit Expressed on Human...