High-throughput label-free molecular fingerprinting flow cytometry.
Carbon Dioxide
/ metabolism
Carbon Isotopes
/ metabolism
Chlorophyceae
/ metabolism
Flow Cytometry
/ instrumentation
Fourier Analysis
High-Throughput Screening Assays
/ instrumentation
Lab-On-A-Chip Devices
Photosynthesis
Reproducibility of Results
Single-Cell Analysis
/ instrumentation
Spectrum Analysis, Raman
/ instrumentation
Vibration
Xanthophylls
/ metabolism
Journal
Science advances
ISSN: 2375-2548
Titre abrégé: Sci Adv
Pays: United States
ID NLM: 101653440
Informations de publication
Date de publication:
01 2019
01 2019
Historique:
received:
29
04
2018
accepted:
06
12
2018
entrez:
13
2
2019
pubmed:
13
2
2019
medline:
22
4
2020
Statut:
epublish
Résumé
Flow cytometry is an indispensable tool in biology for counting and analyzing single cells in large heterogeneous populations. However, it predominantly relies on fluorescent labeling to differentiate cells and, hence, comes with several fundamental drawbacks. Here, we present a high-throughput Raman flow cytometer on a microfluidic chip that chemically probes single live cells in a label-free manner. It is based on a rapid-scan Fourier-transform coherent anti-Stokes Raman scattering spectrometer as an optical interrogator, enabling us to obtain the broadband molecular vibrational spectrum of every single cell in the fingerprint region (400 to 1600 cm
Identifiants
pubmed: 30746443
doi: 10.1126/sciadv.aau0241
pii: aau0241
pmc: PMC6357763
doi:
Substances chimiques
Carbon Isotopes
0
Xanthophylls
0
Carbon Dioxide
142M471B3J
astaxanthine
8XPW32PR7I
Carbon-13
FDJ0A8596D
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
eaau0241Références
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