Dysregulated gene expression in oocysts of Plasmodium berghei LAP mutants.
LCCL
Malaria transmission
Oocyst
Plasmodium berghei
Sporogony
Journal
Molecular and biochemical parasitology
ISSN: 1872-9428
Titre abrégé: Mol Biochem Parasitol
Pays: Netherlands
ID NLM: 8006324
Informations de publication
Date de publication:
04 2019
04 2019
Historique:
received:
16
11
2018
revised:
14
01
2019
accepted:
05
02
2019
pubmed:
13
2
2019
medline:
27
6
2019
entrez:
13
2
2019
Statut:
ppublish
Résumé
Malaria parasite oocysts generate sporozoites by a process termed sporogony. Essential for successful sporogony of Plasmodium berghei in Anopheles stephensi mosquitoes is a complex of six LCCL lectin domain adhesive-like proteins (LAPs). LAP null mutant oocysts undergo growth and mitosis but fail to form sporozoites. At a cytological level, LAP null mutant oocyst development is indistinguishable from its wildtype counterparts for the first week, supporting the hypothesis that LAP null mutant oocysts develop normally before cytokinesis. We show here that LAP1 null mutant oocysts display highly reduced expression of sporozoite proteins and their transcription factors. Our findings indicate that events leading up to the cytokinesis defect in LAP null mutants occur early in oocyst development.
Identifiants
pubmed: 30753856
pii: S0166-6851(18)30232-9
doi: 10.1016/j.molbiopara.2019.02.001
pmc: PMC6452916
pii:
doi:
Substances chimiques
Protozoan Proteins
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
1-5Subventions
Organisme : Medical Research Council
ID : MR/P021611/1
Pays : United Kingdom
Organisme : Wellcome Trust
ID : 088449
Pays : United Kingdom
Organisme : Medical Research Council
ID : MR/P021611
Pays : United Kingdom
Organisme : Biotechnology and Biological Sciences Research Council
ID : BB/M001598
Pays : United Kingdom
Informations de copyright
Copyright © 2019 The Authors. Published by Elsevier B.V. All rights reserved.
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