Effects of lactoferrin on X-ray-induced intestinal injury in Balb/C mice.
Animals
Dose-Response Relationship, Drug
Dose-Response Relationship, Radiation
Interleukin-10
/ blood
Interleukin-6
/ blood
Intestinal Mucosa
/ drug effects
Lactoferrin
/ administration & dosage
Male
Mice
Mice, Inbred BALB C
NF-kappa B
/ metabolism
Radiation Injuries, Experimental
/ metabolism
Radiation-Protective Agents
/ administration & dosage
Tumor Necrosis Factor-alpha
/ blood
Inflammatory response
Intestinal injury
Irradiation
Lactoferrin
Survival rate
Journal
Applied radiation and isotopes : including data, instrumentation and methods for use in agriculture, industry and medicine
ISSN: 1872-9800
Titre abrégé: Appl Radiat Isot
Pays: England
ID NLM: 9306253
Informations de publication
Date de publication:
Apr 2019
Apr 2019
Historique:
received:
28
05
2018
revised:
03
01
2019
accepted:
24
01
2019
pubmed:
13
2
2019
medline:
25
7
2019
entrez:
13
2
2019
Statut:
ppublish
Résumé
Intestinal injury is common after radiotherapy. We aim to investigate the effects of lactoferrin (Lf) on X-ray-induced intestinal injury in Balb/C mice. In assessing animal survival, a total of 40 animals were assigned randomly to 8Gy group, 2 mg Lf+8Gy group, 4 mg Lf+8Gy group, and 6 mg Lf+8Gy group. Mice were administered with Lf intraperitoneally and exposed to single whole-body X-ray irradiation. Lf administration lasted for 3 days. Survival rate was compared among groups. For the observation of intestinal injury, a total of 60 animals were divided randomly into control group, 5Gy group, 2 mg Lf+5Gy group, and 4 mg Lf+5Gy group. Lf was administered once a day. Five mice in each group were randomly sacrificed at days 1, 3, and 9 after irradiation. Fasting blood was used to determine serum levels of pro-inflammatory cytokines IL-6 and TNF-α, and anti-inflammatorycytokine IL-10. Intestinal tissues were collected to examine histological changes and determine the protein expression levels of NF-κB, IKKα and IKKβ. Mean survival time was 4.30 ± 1.34, 4.20 ± 0.71, 5.75 ± 2.44 and 6.70 ± 2.54 days in four groups, respectively, with significantly longer duration in 6 mg Lf+8Gy group than in the 8Gy group. Survival rate was significantly higher in 4 mg Lf+8Gy group and 6 mg Lf+8Gy group, compared with the 8Gy group. For intestinal histology, the radiation-induced injury was considerably improved in the 2 mg Lf+5Gy and 4 mg Lf+5Gy groups. Villus length and its ratio to crypt depth significantly increased in the two Lf intervention groups. Compared with 5Gy group, serum levels of IL-6 and TNF-α significantly decreased in the two Lf intervention groups at days 3 and 9. Furthermore, Lf significantly reduced the radiation-induced expression of IKKα/β and NF-κB at day 3 and/or day 9. Lf extended the survival time of radiated mice and improved intestinal injury by decreasing inflammatory cytokines and downregulating NF-κB expression.
Identifiants
pubmed: 30753988
pii: S0969-8043(18)30486-X
doi: 10.1016/j.apradiso.2019.01.014
pii:
doi:
Substances chimiques
IL10 protein, mouse
0
Interleukin-6
0
NF-kappa B
0
Radiation-Protective Agents
0
Tnf protein, mouse
0
Tumor Necrosis Factor-alpha
0
interleukin-6, mouse
0
Interleukin-10
130068-27-8
Lactoferrin
EC 3.4.21.-
Types de publication
Journal Article
Langues
eng
Pagination
72-77Informations de copyright
Copyright © 2019 Elsevier Ltd. All rights reserved.