Long-term in vitro 3D hydrogel co-culture model of inflammatory bowel disease.
ADAMTS1 Protein
/ metabolism
Caco-2 Cells
Caspase 3
/ metabolism
Cell Hypoxia
/ drug effects
Coculture Techniques
/ methods
HT29 Cells
Humans
Hydrogels
/ chemistry
Inflammatory Bowel Diseases
/ metabolism
Interleukin-1beta
/ pharmacology
Mucin 5AC
/ metabolism
Mucin-2
/ metabolism
Tumor Necrosis Factor-alpha
/ pharmacology
Zonula Occludens-1 Protein
/ metabolism
Journal
Scientific reports
ISSN: 2045-2322
Titre abrégé: Sci Rep
Pays: England
ID NLM: 101563288
Informations de publication
Date de publication:
12 02 2019
12 02 2019
Historique:
received:
01
08
2018
accepted:
13
12
2018
entrez:
14
2
2019
pubmed:
14
2
2019
medline:
1
9
2020
Statut:
epublish
Résumé
The in vitro study of the pathogenesis of inflammatory bowel disease (IBD) requires a cell model which closely reflects the characteristics of the in vivo intestinal epithelium. This study aimed to investigate the application of L-pNIPAM hydrogel as a scaffold to develop a long-term 3D co-culture model of Caco-2 and HT29-MTX cells under conditions analogous to inflammation, to determine its potential use in studying IBD. Monocultures and co-cultures were layered on L-pNIPAM hydrogel scaffolds and maintained under dynamic culture conditions for up to 12 weeks. Treatments with IL-1β, TNFα, and hypoxia for 1 week were used to create an inflammatory environment. Following prolonged culture, the metabolic activity of Caco-2 monoculture and 90% Caco-2/10% HT29-MTX co-cultures on L-pNIPAM hydrogels were increased, and finger-like structures, similar in appearance to villi were observed. Following treatment with IL-1β, TNFα and hypoxia, ALP and ZO-1 were decreased, MUC2 increased, and MUC5AC remained unchanged. ADAMTS1 was increased in response to hypoxia. Caspase 3 expression was increased in response to TNFα and hypoxic conditions. In conclusion, L-pNIPAM hydrogel supported long-term co-culture within a 3D model. Furthermore, stimulation with factors seen during inflammation recapitulated features seen during IBD.
Identifiants
pubmed: 30755679
doi: 10.1038/s41598-019-38524-8
pii: 10.1038/s41598-019-38524-8
pmc: PMC6372635
doi:
Substances chimiques
Hydrogels
0
Interleukin-1beta
0
MUC2 protein, human
0
MUC5AC protein, human
0
Mucin 5AC
0
Mucin-2
0
TJP1 protein, human
0
Tumor Necrosis Factor-alpha
0
Zonula Occludens-1 Protein
0
Caspase 3
EC 3.4.22.-
ADAMTS1 Protein
EC 3.4.24.-
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
1812Références
Gastroenterology. 1999 Oct;117(4):814-22
pubmed: 10500063
J Pharm Sci. 2000 Jan;89(1):63-75
pubmed: 10664539
Inflamm Res. 2000 Apr;49(4):162-9
pubmed: 10858016
Int J Biochem Cell Biol. 2001 Jan;33(1):33-44
pubmed: 11167130
J Interferon Cytokine Res. 2001 Feb;21(2):93-8
pubmed: 11244573
J Pharmacol Toxicol Methods. 2000 Jul-Aug;44(1):301-12
pubmed: 11274897
Nat Rev Cancer. 2002 Mar;2(3):161-74
pubmed: 11990853
Toxicol In Vitro. 2003 Oct-Dec;17(5-6):761-7
pubmed: 14599474
Gut. 2004 Dec;53(12):1724-5
pubmed: 15542502
Cell Physiol Biochem. 2005;15(1-4):29-40
pubmed: 15665513
Dig Dis Sci. 1992 Jun;37(6):818-26
pubmed: 1587185
Arthritis Rheum. 2005 May;52(5):1451-60
pubmed: 15880812
Oncogene. 2006 Apr 13;25(16):2379-92
pubmed: 16369494
BMC Cell Biol. 2006 May 02;7:20
pubmed: 16670004
Hum Pathol. 2007 Jan;38(1):86-94
pubmed: 16949904
Gastroenterology. 2006 Oct;131(4):1153-63
pubmed: 17030185
J Immunol. 2007 Apr 1;178(7):4641-9
pubmed: 17372023
Transl Res. 2007 Apr;149(4):173-86
pubmed: 17383591
Proc Natl Acad Sci U S A. 2008 Mar 4;105(9):3551-6
pubmed: 18292227
Inflamm Bowel Dis. 2009 Jan;15(1):100-13
pubmed: 18623167
J Nutr Biochem. 2009 Jul;20(7):494-502
pubmed: 18715773
Cytokine. 1991 May;3(3):189-94
pubmed: 1883957
Curr Drug Metab. 2008 Nov;9(9):893-900
pubmed: 18991586
Nature. 2009 May 14;459(7244):262-5
pubmed: 19329995
Zhonghua Shao Shang Za Zhi. 2009 Feb;25(1):57-60
pubmed: 19588764
J Pharmacol Toxicol Methods. 2010 May-Jun;61(3):334-42
pubmed: 20159047
Nat Rev Gastroenterol Hepatol. 2010 May;7(5):281-7
pubmed: 20368740
Toxicol In Vitro. 2010 Aug;24(5):1441-9
pubmed: 20406675
Braz J Med Biol Res. 2010 Apr;43(4):330-7
pubmed: 20445948
Curr Gastroenterol Rep. 2010 Oct;12(5):340-8
pubmed: 20683682
Mol Pharm. 2010 Dec 6;7(6):2103-19
pubmed: 20809575
Am J Pathol. 2010 Nov;177(5):2310-22
pubmed: 21048223
Proc Natl Acad Sci U S A. 2011 Mar 29;108(13):5396-401
pubmed: 21402942
Cell Mol Life Sci. 2011 Nov;68(22):3635-41
pubmed: 21947475
Toxicol In Vitro. 2012 Dec;26(8):1247-51
pubmed: 22123491
Biotechnol Bioeng. 2012 Sep;109(9):2173-8
pubmed: 22488418
J Exp Med. 2012 Aug 27;209(9):1595-609
pubmed: 22891275
Acta Med Okayama. 2012;66(5):399-407
pubmed: 23093058
World J Gastroenterol. 2012 Nov 7;18(41):5848-61
pubmed: 23139600
Eur J Pharm Biopharm. 2013 Apr;83(3):427-35
pubmed: 23159710
Tissue Eng Part C Methods. 2013 Sep;19(9):708-19
pubmed: 23350801
Lab Chip. 2013 Apr 21;13(8):1586-92
pubmed: 23443975
Gastroenterol Res Pract. 2013;2013:431231
pubmed: 23737764
Rev Inst Med Trop Sao Paulo. 2013 Sep-Oct;55(5):341-6
pubmed: 24037289
Biotechnol Bioeng. 2014 Jun;111(6):1222-32
pubmed: 24390638
Eur J Pharm Biopharm. 2014 Jul;87(2):290-8
pubmed: 24704198
Mol Pharm. 2014 Jul 7;11(7):2030-9
pubmed: 24798584
Integr Biol (Camb). 2014 Dec;6(12):1122-31
pubmed: 25200891
Intest Res. 2015 Jan;13(1):11-8
pubmed: 25691839
PLoS One. 2015 Mar 18;10(3):e0121209
pubmed: 25786261
Mediators Inflamm. 2015;2015:964131
pubmed: 25948887
Am J Physiol Cell Physiol. 2015 Sep 15;309(6):C350-60
pubmed: 26179603
Sci Rep. 2015 Sep 16;5:13708
pubmed: 26374193
Transl Res. 2016 Mar;169:19-30.e1
pubmed: 26589109
PLoS One. 2016 Jan 05;11(1):e0146103
pubmed: 26731748
Mol Cell Pediatr. 2016 Dec;3(1):1
pubmed: 26812949
Mol Cell Pediatr. 2016 Dec;3(1):14
pubmed: 27002817
World J Gastroenterol. 2016 Mar 21;22(11):3117-26
pubmed: 27003989
Annu Rev Pathol. 2016 May 23;11:77-100
pubmed: 27193451
Mol Pharm. 2017 Apr 3;14(4):1264-1270
pubmed: 28263609
Mediators Inflamm. 2017;2017:4810258
pubmed: 28420941
Yakugaku Zasshi. 2017;137(7):811-814
pubmed: 28674292
Int J Mol Sci. 2017 Jul 20;18(7):null
pubmed: 28726765
Acta Biomater. 2017 Oct 15;62:128-143
pubmed: 28859901
Tissue Eng Part B Rev. 2018 Apr;24(2):98-111
pubmed: 28922991
Cell Mol Gastroenterol Hepatol. 2017 Nov 02;5(1):51-60
pubmed: 29204508
Oncotarget. 2018 Apr 6;9(26):18277-18295
pubmed: 29719605
Digestion. 1983;27(2):70-4
pubmed: 6414866
Br Med J (Clin Res Ed). 1982 Jul 3;285(6334):20-1
pubmed: 6805795
Gastroenterology. 1995 Oct;109(4):1344-67
pubmed: 7557106
Gastroenterology. 1994 Jun;106(6):1455-66
pubmed: 8194690
Clin Exp Immunol. 1993 Oct;94(1):174-81
pubmed: 8403503
Gut. 1996 Feb;38(2):240-2
pubmed: 8801204
J Immunol. 1996 Nov 1;157(9):4159-65
pubmed: 8892653
J Pharm Sci. 1996 Oct;85(10):1070-6
pubmed: 8897273
J Membr Biol. 1998 Feb 1;161(3):263-74
pubmed: 9493132
World J Surg. 1998 Apr;22(4):382-9
pubmed: 9523521