Activation of Protein Kinase A Stimulates SUMOylation, Expression, and Transport Activity of Organic Anion Transporter 3.


Journal

The AAPS journal
ISSN: 1550-7416
Titre abrégé: AAPS J
Pays: United States
ID NLM: 101223209

Informations de publication

Date de publication:
13 02 2019
Historique:
received: 15 11 2018
accepted: 22 01 2019
entrez: 15 2 2019
pubmed: 15 2 2019
medline: 15 2 2020
Statut: epublish

Résumé

Organic anion transporter 3 (OAT3) plays a vital role in removing a broad variety of anionic drugs from kidney, thus avoiding their possible toxicity in the body. We earlier established that activation of protein kinase C (PKC) enhances OAT3 ubiquitination, which promotes OAT3 internalization from the cell plasma membrane to intracellular endosomes and consequent degradation. As a result, OAT3 expression and transport activity are reduced. In the current study, we discovered that protein kinase A (PKA) had an opposite effect to PKC on the regulation of OAT3. We showed that activation of PKA by Bt2-cAMP stimulated OAT3 transport activity, which was largely caused by an enhanced plasma membrane expression of the transporter, kinetically reflected as an augmented maximal transport velocity V

Identifiants

pubmed: 30761470
doi: 10.1208/s12248-019-0303-4
pii: 10.1208/s12248-019-0303-4
pmc: PMC6647857
mid: NIHMS1041849
doi:

Substances chimiques

IGF1 protein, human 0
Organic Anion Transporters, Sodium-Independent 0
SUMO-1 Protein 0
SUMO1 protein, human 0
SUMO2 protein, human 0
SUMO3 protein, human 0
Small Ubiquitin-Related Modifier Proteins 0
Ubiquitins 0
organic anion transport protein 3 0
Insulin-Like Growth Factor I 67763-96-6
Ubiquitin-Conjugating Enzymes EC 2.3.2.23
Cyclic AMP-Dependent Protein Kinases EC 2.7.11.11
ubiquitin-conjugating enzyme UBC9 EC 6.3.2.-

Types de publication

Journal Article Research Support, N.I.H., Extramural

Langues

eng

Sous-ensembles de citation

IM

Pagination

30

Subventions

Organisme : NIGMS NIH HHS
ID : R01 GM079123
Pays : United States
Organisme : NIGMS NIH HHS
ID : R01 GM097000
Pays : United States

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Auteurs

Haoxun Wang (H)

Department of Pharmaceutics, Rutgers, The State University of New Jersey, 160 Frelinghuysen Road, Piscataway, New Jersey, 08854, USA.

Jinghui Zhang (J)

Department of Pharmaceutics, Rutgers, The State University of New Jersey, 160 Frelinghuysen Road, Piscataway, New Jersey, 08854, USA.

Guofeng You (G)

Department of Pharmaceutics, Rutgers, The State University of New Jersey, 160 Frelinghuysen Road, Piscataway, New Jersey, 08854, USA. gyou@pharmacy.rutgers.edu.

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Classifications MeSH