PMab-219: A monoclonal antibody for the immunohistochemical analysis of horse podoplanin.
CBIS, Cell-Based Immunization and Screening
CHO, Chinese hamster ovary
CLEC-2, C-type lectin-like receptor-2
DAB, 3,3'-diaminobenzidine tetrahydrochloride
ELISA, enzyme-linked immunosorbent assay
Horse podoplanin
PBS, phosphate-buffered saline
PDPN
PDPN, podoplanin
PMab-219
PVDF, polyvinylidene difluoride
SDS, sodium dodecyl sulfate
hPDPN, human podoplanin
horPDPN, horse podoplanin
mAb, monoclonal antibody
Journal
Biochemistry and biophysics reports
ISSN: 2405-5808
Titre abrégé: Biochem Biophys Rep
Pays: Netherlands
ID NLM: 101660999
Informations de publication
Date de publication:
Jul 2019
Jul 2019
Historique:
received:
02
11
2018
revised:
08
12
2018
accepted:
27
01
2019
entrez:
16
2
2019
pubmed:
16
2
2019
medline:
16
2
2019
Statut:
epublish
Résumé
Monoclonal antibodies (mAbs) against human, mouse, rat, rabbit, dog, cat, and bovine podoplanin (PDPN), a lymphatic endothelial cell marker, have been established in our previous studies. However, mAbs against horse PDPN (horPDPN), which are useful for immunohistochemical analysis, remain to be developed. In the present study, mice were immunized with horPDPN-overexpressing Chinese hamster ovary (CHO)-K1 cells (CHO/horPDPN), and hybridomas producing mAbs against horPDPN were screened using flow cytometry. One of the mAbs, PMab-219 (IgG
Identifiants
pubmed: 30766925
doi: 10.1016/j.bbrep.2019.01.009
pii: S2405-5808(18)30268-1
pii: 100616
pmc: PMC6360987
doi:
Types de publication
Journal Article
Langues
eng
Pagination
100616Références
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