Intracellular dynamics of actin affects Borna disease virus replication in the nucleus.
Actin
Borna disease virus (BoDV)
Exportin 6
Importin 9
Transcription
Journal
Virus research
ISSN: 1872-7492
Titre abrégé: Virus Res
Pays: Netherlands
ID NLM: 8410979
Informations de publication
Date de publication:
02 04 2019
02 04 2019
Historique:
received:
15
12
2018
revised:
09
02
2019
accepted:
11
02
2019
pubmed:
16
2
2019
medline:
25
4
2019
entrez:
16
2
2019
Statut:
ppublish
Résumé
Borna disease virus (BoDV) is a nonsegmented, negative-strand RNA virus that uniquely replicates and establishes persistent infection in cell nucleus. Recent studies have demonstrated the presence of actin in the nucleus and its role in intranuclear phenomena such as transcription and DNA repair. Although nuclear actin is involved in the life cycle of some intranuclear DNA viruses, the interaction between BoDV and nuclear actin has not been reported. In this study, we show that the inhibition of the nucleocytoplasmic transport of actin affects the replication of BoDV in the nucleus. The knockdown of a nuclear export factor of actin, exportin 6, results in the induction of structural aberration in intranuclear viral factories of BoDV. Furthermore, the inhibition of the nuclear export of actin promotes accumulation of viral matrix protein in the cytoplasm and periphery of the infected cells. These results suggest that the dynamics of actin affect the replication of BoDV by disturbing the structure of viral factories in the nucleus.
Identifiants
pubmed: 30769121
pii: S0168-1702(18)30758-5
doi: 10.1016/j.virusres.2019.02.004
pii:
doi:
Substances chimiques
Actins
0
Karyopherins
0
XPO6 protein, human
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
179-183Informations de copyright
Copyright © 2019 Elsevier B.V. All rights reserved.