Curcumin prevents high glucose damage in retinal pigment epithelial cells through ERK1/2-mediated activation of the Nrf2/HO-1 pathway.
Apoptosis
/ drug effects
Cell Survival
/ drug effects
Curcumin
/ pharmacology
Epithelial Cells
/ drug effects
Glucose
/ pharmacology
Heme Oxygenase-1
/ metabolism
Humans
MAP Kinase Signaling System
/ drug effects
NF-E2-Related Factor 2
/ drug effects
Oxidative Stress
/ drug effects
Reactive Oxygen Species
/ metabolism
Retinal Pigment Epithelium
/ metabolism
apoptosis
curcumin
diabetic retinopathy (DR)
heme oxygenase-1 (HO-1)
retinal pigment epithelial (RPE) cells
Journal
Journal of cellular physiology
ISSN: 1097-4652
Titre abrégé: J Cell Physiol
Pays: United States
ID NLM: 0050222
Informations de publication
Date de publication:
08 2019
08 2019
Historique:
received:
08
11
2018
revised:
28
01
2019
accepted:
30
01
2019
pubmed:
17
2
2019
medline:
2
6
2020
entrez:
17
2
2019
Statut:
ppublish
Résumé
To study the effects of curcumin on human retinal pigment epithelial (RPE) cells exposed to high glucose (HG) insult, we performed in vitro studies on RPE cells cultured both in normal and HG conditions to assess the effects of curcumin on the cell viability, nuclear factor erythroid 2-related factor 2 (Nrf2) expression, HO-1 activity, and ERK1/2 expression. RPE cells exposed to HG insult were treated with curcumin. The cell viability, apoptosis, HO-1 activity, ERK, and Nrf2 expression were evaluated. The data indicated that treatment with curcumin caused a significant decrease in terms of apoptosis. Further, curcumin was able to induce HO-1 expression via Nrf2 activation and counteracts the damage elicited by HG. The present study demonstrated that curcumin provides protection against HG-induced damage in RPE cells through the activation of Nrf2/HO-1 signaling that involves the ERK pathway, suggesting that curcumin may have therapeutic value in the treatment of diabetic retinopathy.
Substances chimiques
NF-E2-Related Factor 2
0
NFE2L2 protein, human
0
Reactive Oxygen Species
0
Heme Oxygenase-1
EC 1.14.14.18
Curcumin
IT942ZTH98
Glucose
IY9XDZ35W2
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
17295-17304Informations de copyright
© 2019 Wiley Periodicals, Inc.