Functional Heatmap: an automated and interactive pattern recognition tool to integrate time with multi-omics assays.


Journal

BMC bioinformatics
ISSN: 1471-2105
Titre abrégé: BMC Bioinformatics
Pays: England
ID NLM: 100965194

Informations de publication

Date de publication:
15 Feb 2019
Historique:
received: 01 10 2018
accepted: 28 01 2019
entrez: 17 2 2019
pubmed: 17 2 2019
medline: 14 3 2019
Statut: epublish

Résumé

Life science research is moving quickly towards large-scale experimental designs that are comprised of multiple tissues, time points, and samples. Omic time-series experiments offer answers to three big questions: what collective patterns do most analytes follow, which analytes follow an identical pattern or synchronize across multiple cohorts, and how do biological functions evolve over time. Existing tools fall short of robustly answering and visualizing all three questions in a unified interface. Functional Heatmap offers time-series data visualization through a Master Panel page, and Combined page to answer each of the three time-series questions. It dissects the complex multi-omics time-series readouts into patterned clusters with associated biological functions. It allows users to identify a cascade of functional changes over a time variable. Inversely, Functional Heatmap can compare a pattern with specific biology respond to multiple experimental conditions. All analyses are interactive, searchable, and exportable in a form of heatmap, line-chart, or text, and the results are easy to share, maintain, and reproduce on the web platform. Functional Heatmap is an automated and interactive tool that enables pattern recognition in time-series multi-omics assays. It significantly reduces the manual labour of pattern discovery and comparison by transferring statistical models into visual clues. The new pattern recognition feature will help researchers identify hidden trends driven by functional changes using multi-tissues/conditions on a time-series fashion from omic assays.

Sections du résumé

BACKGROUND BACKGROUND
Life science research is moving quickly towards large-scale experimental designs that are comprised of multiple tissues, time points, and samples. Omic time-series experiments offer answers to three big questions: what collective patterns do most analytes follow, which analytes follow an identical pattern or synchronize across multiple cohorts, and how do biological functions evolve over time. Existing tools fall short of robustly answering and visualizing all three questions in a unified interface.
RESULTS RESULTS
Functional Heatmap offers time-series data visualization through a Master Panel page, and Combined page to answer each of the three time-series questions. It dissects the complex multi-omics time-series readouts into patterned clusters with associated biological functions. It allows users to identify a cascade of functional changes over a time variable. Inversely, Functional Heatmap can compare a pattern with specific biology respond to multiple experimental conditions. All analyses are interactive, searchable, and exportable in a form of heatmap, line-chart, or text, and the results are easy to share, maintain, and reproduce on the web platform.
CONCLUSIONS CONCLUSIONS
Functional Heatmap is an automated and interactive tool that enables pattern recognition in time-series multi-omics assays. It significantly reduces the manual labour of pattern discovery and comparison by transferring statistical models into visual clues. The new pattern recognition feature will help researchers identify hidden trends driven by functional changes using multi-tissues/conditions on a time-series fashion from omic assays.

Identifiants

pubmed: 30770734
doi: 10.1186/s12859-019-2657-0
pii: 10.1186/s12859-019-2657-0
pmc: PMC6377781
doi:

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Pagination

81

Subventions

Organisme : CCR NIH HHS
ID : HHSN261200800001C
Pays : United States
Organisme : NCI NIH HHS
ID : HHSN261200800001E
Pays : United States
Organisme : Medical Research and Materiel Command
ID : 09284002

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Auteurs

Joshua R Williams (JR)

Advanced Biomedical Computational Science, Frederick National Laboratory for Cancer Research sponsored by the National Cancer Institute, Frederick, MD, 21702-5010, USA.
Integrative Systems Biology Program, US Army Center for Environmental Health Research, Fort Detrick, Frederick, MD, 21702-5010, USA.

Ruoting Yang (R)

Advanced Biomedical Computational Science, Frederick National Laboratory for Cancer Research sponsored by the National Cancer Institute, Frederick, MD, 21702-5010, USA.
Integrative Systems Biology Program, US Army Center for Environmental Health Research, Fort Detrick, Frederick, MD, 21702-5010, USA.

John L Clifford (JL)

Integrative Systems Biology Program, US Army Center for Environmental Health Research, Fort Detrick, Frederick, MD, 21702-5010, USA.

Daniel Watson (D)

Advanced Biomedical Computational Science, Frederick National Laboratory for Cancer Research sponsored by the National Cancer Institute, Frederick, MD, 21702-5010, USA.

Ross Campbell (R)

Advanced Biomedical Computational Science, Frederick National Laboratory for Cancer Research sponsored by the National Cancer Institute, Frederick, MD, 21702-5010, USA.
Integrative Systems Biology Program, US Army Center for Environmental Health Research, Fort Detrick, Frederick, MD, 21702-5010, USA.

Derese Getnet (D)

Integrative Systems Biology Program, US Army Center for Environmental Health Research, Fort Detrick, Frederick, MD, 21702-5010, USA.

Raina Kumar (R)

Advanced Biomedical Computational Science, Frederick National Laboratory for Cancer Research sponsored by the National Cancer Institute, Frederick, MD, 21702-5010, USA.
Integrative Systems Biology Program, US Army Center for Environmental Health Research, Fort Detrick, Frederick, MD, 21702-5010, USA.

Rasha Hammamieh (R)

Integrative Systems Biology Program, US Army Center for Environmental Health Research, Fort Detrick, Frederick, MD, 21702-5010, USA.

Marti Jett (M)

Integrative Systems Biology Program, US Army Center for Environmental Health Research, Fort Detrick, Frederick, MD, 21702-5010, USA. marti.jett-tilton.civ@mail.mil.

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Classifications MeSH